益肾壮骨颗粒剂对人工关节生物因素松动的影响(1)
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(1.杭州市中医院，浙江 杭州 310007；2.平湖市中医院，浙江 平湖 314200)
摘 要：目的： 探讨益肾壮骨颗粒剂对人工关节生物因素松动的影响。方法：随机选择准备行人工髋关节置换术患者20例。采集外周血，分离单个核细胞，每例标本分成5份。A组：仅单个核细胞，为正常对照组；B组：单个核细胞+骨水泥微粒，为模型组；C组：单个核细胞+骨水泥微粒+益肾壮骨低剂量组(3mg·mL-1)；D组：单个核细胞+骨水泥微粒+益肾壮骨中剂量组(6mg·mL-1)；E组：单个核细胞+骨水泥微粒+益肾壮骨高剂量组(12mg·mL-1)。培养48 h后，用ELISA测定细胞上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量。结果：模型组的TNF-α和IL-6含量均明显高于正常对照组(P<0.01)；单个核细胞+骨水泥微粒+益肾壮骨各剂量组的TNF-α和IL-6含量明显低于模型组(P<0.05)。结论：骨水泥微粒可刺激单个核细胞分泌TNF-α和IL-6，而益肾壮骨颗粒剂能有效地抑制单个核细胞分泌TNF-α和IL-6。益肾壮骨颗粒剂对人工关节生物因素松动方面有一定的防治作用。
关键词：益肾壮骨颗粒剂；人工关节；假体失效；骨溶解
中图分类号：R318.17文献标识码：A文章编号：1673-7717(2011)03-0520-03
TheCytokine Study of Yishenzhuanggu'sEffect on the Artificial Joint Loosening
CHENG Yi1, CHEN Jun-bo2 , HUANG Hai,ZHU Hang1,ZHANG Yu-ming1
(1.Hangzhou Hospital of TCM , Hangzhou 310007,Zhejiang,China；
2.Pinghu Hospital of TCM,Pinghu 314200,Zhejiang,China)
Abstract:Objective:To explore the effects ofYiShenZhuangGu on aseptic cytokine of loosening of prothesis in vitro. Methods:From 20 patients，blood sample were taken, whose mononuclear cells were isolate and then divided into five groups.Group A:only mononuclear cells, which was control group;group B:mononuclear cells + Poly methyl methacrylate particles;Group C:mononuclear cells + Poly methyl methacrylate particles + YiShenZhuangGu (3mg·mL-1);Group D:mononuclear cells + Poly methyl methacrylate particles + YiShenZhuangGu (6mg·mL-1);Group E:mononuclear cells + Poly methyl methacrylateparticles + YiShenZhuangGu (12mg·mL-1).They were cultured for 48 hours, and the a mount of osteolytic factors such as TNF-a and IL-6 of each group was determined. Results: The levels of TNF-α and IL-6 were much higher in group B than A, C, D and E(P<0.01).The levels of TNF-α and IL-6 were much lower in group C, D and E than B(P<0.05).Conclusion: It shows the Poly methyl methacrylate particles could stimulate mononuclear cells to excrete TNF-α and IL-6, which can be inhibited by YiShenZhuangGu.By inhibiting bone resorption associated with the particles and TNF-α and IL-6, YiShenZhuangGu may have clinical utility in the prevention and treatment of aseptic loosening of prothesis. ......
(1.杭州市中医院，浙江 杭州 310007；2.平湖市中医院，浙江 平湖 314200)
摘 要：目的： 探讨益肾壮骨颗粒剂对人工关节生物因素松动的影响。方法：随机选择准备行人工髋关节置换术患者20例。采集外周血，分离单个核细胞，每例标本分成5份。A组：仅单个核细胞，为正常对照组；B组：单个核细胞+骨水泥微粒，为模型组；C组：单个核细胞+骨水泥微粒+益肾壮骨低剂量组(3mg·mL-1)；D组：单个核细胞+骨水泥微粒+益肾壮骨中剂量组(6mg·mL-1)；E组：单个核细胞+骨水泥微粒+益肾壮骨高剂量组(12mg·mL-1)。培养48 h后，用ELISA测定细胞上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量。结果：模型组的TNF-α和IL-6含量均明显高于正常对照组(P<0.01)；单个核细胞+骨水泥微粒+益肾壮骨各剂量组的TNF-α和IL-6含量明显低于模型组(P<0.05)。结论：骨水泥微粒可刺激单个核细胞分泌TNF-α和IL-6，而益肾壮骨颗粒剂能有效地抑制单个核细胞分泌TNF-α和IL-6。益肾壮骨颗粒剂对人工关节生物因素松动方面有一定的防治作用。
关键词：益肾壮骨颗粒剂；人工关节；假体失效；骨溶解
中图分类号：R318.17文献标识码：A文章编号：1673-7717(2011)03-0520-03
TheCytokine Study of Yishenzhuanggu'sEffect on the Artificial Joint Loosening
CHENG Yi1, CHEN Jun-bo2 , HUANG Hai,ZHU Hang1,ZHANG Yu-ming1
(1.Hangzhou Hospital of TCM , Hangzhou 310007,Zhejiang,China；
2.Pinghu Hospital of TCM,Pinghu 314200,Zhejiang,China)
Abstract:Objective:To explore the effects ofYiShenZhuangGu on aseptic cytokine of loosening of prothesis in vitro. Methods:From 20 patients，blood sample were taken, whose mononuclear cells were isolate and then divided into five groups.Group A:only mononuclear cells, which was control group;group B:mononuclear cells + Poly methyl methacrylate particles;Group C:mononuclear cells + Poly methyl methacrylate particles + YiShenZhuangGu (3mg·mL-1);Group D:mononuclear cells + Poly methyl methacrylate particles + YiShenZhuangGu (6mg·mL-1);Group E:mononuclear cells + Poly methyl methacrylateparticles + YiShenZhuangGu (12mg·mL-1).They were cultured for 48 hours, and the a mount of osteolytic factors such as TNF-a and IL-6 of each group was determined. Results: The levels of TNF-α and IL-6 were much higher in group B than A, C, D and E(P<0.01).The levels of TNF-α and IL-6 were much lower in group C, D and E than B(P<0.05).Conclusion: It shows the Poly methyl methacrylate particles could stimulate mononuclear cells to excrete TNF-α and IL-6, which can be inhibited by YiShenZhuangGu.By inhibiting bone resorption associated with the particles and TNF-α and IL-6, YiShenZhuangGu may have clinical utility in the prevention and treatment of aseptic loosening of prothesis. ......
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