抗毒扶正1\2号方提取液体外抗SRS病毒细胞活性作用(1)
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(上海中医药大学，上海 201203)
摘 要：目的： 探讨抗毒扶正1、2号方提取液对SRS病毒细胞增殖的抑制作用，为艾滋病临床治疗提供实验依据。方法： 将抗毒扶正1、2号方提取液倍比稀释若干浓度，加入SRS细胞体系。以[3H]-TdR掺入法和RT-PCR法观测抗毒扶正1、2号方提取液对SRS细胞增殖的影响。结果： 抗毒扶正1、2号方提取液组cpm值均较阴性对照组有所降低，SRSmRNA表达较阴性对照组有所降低。结论：抗毒扶正1、2号方提取液均有抑制SRS病毒细胞增殖的作用，且随药物浓度的升高，抑制作用也逐渐增强。提示抗毒扶正1、2号方对SRS病毒细胞具杀伤作用，对艾滋病临床治疗中药抗毒扶正方的筛选具实用价值。
关键词：抗毒扶正；[3H]-TdR掺入法；细胞增殖；温病
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)03-0566-03
Experimental Study on the Activity of SRS Virus Cell Intervened by
Antitoxic and Fuzheng 1,2 Recipe Extract in Vitro
TU Yan-jie,GUO Yong-jie,YANG Ai-dong,YANG Yang,WU Zhong-hua,WANG Shuang
(Shanghai University of TCM,Shanghai 201203,China)
Abstract:Objective：To approach the effect of Antitoxic and Fuzheng 1,2 recipe extracts on SRS cell's proliferation ，which is in order to provide scientific base for TCM screening in Clinical treatment of AIDS. Methods：Antitoxic and Fuzheng 1,2 recipe extracts are diluted into different concentrations and added into the SRS virus cell. The SRS cell's proliferated suppression ratio are detected by [3H]-TdR incorporation method, and SRS virus's mRNA express are determined by RT-PCR method. Results：The cpm value and the SRS virus's mRNA express in antitoxic and Fuzheng 1,2 recipe extracts are lower than the natural control group. Conclusions：Antitoxic and Fuzheng 1,2 recipe extracts both have the inhibiting effect on the SRS virus cell's proliferation.With the raise up of drug concentration,the inhibiting effect are raise up too.
Key words:Antitoxic and Fuzheng;[3H]-TdR incorporation;cell's proliferation；warm diseases
收稿日期：2010-10-25
基金项目：上海高校选拔培养优秀青年教师科研专项基金项目[沪教委人(2007)68号]
作者简介：屠燕捷(1980-) ......
(上海中医药大学，上海 201203)
摘 要：目的： 探讨抗毒扶正1、2号方提取液对SRS病毒细胞增殖的抑制作用，为艾滋病临床治疗提供实验依据。方法： 将抗毒扶正1、2号方提取液倍比稀释若干浓度，加入SRS细胞体系。以[3H]-TdR掺入法和RT-PCR法观测抗毒扶正1、2号方提取液对SRS细胞增殖的影响。结果： 抗毒扶正1、2号方提取液组cpm值均较阴性对照组有所降低，SRSmRNA表达较阴性对照组有所降低。结论：抗毒扶正1、2号方提取液均有抑制SRS病毒细胞增殖的作用，且随药物浓度的升高，抑制作用也逐渐增强。提示抗毒扶正1、2号方对SRS病毒细胞具杀伤作用，对艾滋病临床治疗中药抗毒扶正方的筛选具实用价值。
关键词：抗毒扶正；[3H]-TdR掺入法；细胞增殖；温病
中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)03-0566-03
Experimental Study on the Activity of SRS Virus Cell Intervened by
Antitoxic and Fuzheng 1,2 Recipe Extract in Vitro
TU Yan-jie,GUO Yong-jie,YANG Ai-dong,YANG Yang,WU Zhong-hua,WANG Shuang
(Shanghai University of TCM,Shanghai 201203,China)
Abstract:Objective：To approach the effect of Antitoxic and Fuzheng 1,2 recipe extracts on SRS cell's proliferation ，which is in order to provide scientific base for TCM screening in Clinical treatment of AIDS. Methods：Antitoxic and Fuzheng 1,2 recipe extracts are diluted into different concentrations and added into the SRS virus cell. The SRS cell's proliferated suppression ratio are detected by [3H]-TdR incorporation method, and SRS virus's mRNA express are determined by RT-PCR method. Results：The cpm value and the SRS virus's mRNA express in antitoxic and Fuzheng 1,2 recipe extracts are lower than the natural control group. Conclusions：Antitoxic and Fuzheng 1,2 recipe extracts both have the inhibiting effect on the SRS virus cell's proliferation.With the raise up of drug concentration,the inhibiting effect are raise up too.
Key words:Antitoxic and Fuzheng;[3H]-TdR incorporation;cell's proliferation；warm diseases
收稿日期：2010-10-25
基金项目：上海高校选拔培养优秀青年教师科研专项基金项目[沪教委人(2007)68号]
作者简介：屠燕捷(1980-) ......
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