正交设计优选新疆紫花苜蓿总黄酮提取工艺
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(1.新疆医科大学中医学院，新疆 乌鲁木齐 830011，2.北京中医药大学中药学院，北京 100102)
摘 要：目的：研究新疆紫花苜蓿中总黄酮的提取工艺。方法：以总黄酮含量为考察指标，采用正交实验法，考察加醇量、超声提取时间、超声功率、乙醇浓度4个因素对提取工艺的影响。结果：最佳提取工艺为A2B1C1D2，结合生产实际，确定最佳工艺为70%乙醇超声提取20分钟，超声功率为60%，加乙醇量为30倍量。结论：正交实验法优选新疆紫花苜蓿总黄酮的提取工艺简便、快速、准确。
关键词：新疆紫花苜蓿；总黄酮；提取工艺
中图分类号：R284.2文献标识码：A文章编号：1673-7717(2011)03-0646-02
Study on Extracting Flavonoid Compounds from Alfalfa of Xinjiang
LIU Hong-bing1,YAN Xue-hua1,TIAN Shu-ge1,LEI Hai-min2
(1.Xinjiang Medical University,Urumqi 830011,Xinjiang, China;2.Beijing University of Chinese Medicine, Beijing 100102, China)
Abstract:Objective:Study on the extracting flavonoid compounds from Alfalfa of Xinjiang.Methods:With flavonoid content as the inspection indicators, orthogonal test was used to investigate the influence of four extrac-ting factors(ethanol volume, ultrasonic time，ultrasonic power and ethanol consistence)on the extracting process.Results:The optimum extracting process was A2B1C1D2, ie The ethanol volume being 30 times of the weight of medicinal material，extracted for 20 minutes under the power of 60% with ultrasonic extraction,and 70% ethanol consistence.Conclusion:Optimization of extracting process for Alfalfa by orthogonal test is a simple, quick and accuratemethod.
Key words:alfalfa；flavonoid；extracting technology
紫花苜蓿(Medicago sativa L.)为豆科(Leguminosae)苜蓿属(Medicago L.)植物。属重要的饲料植物，素有“牧草之王”的美称，广泛分布于世界各地。紫花苜蓿在我国作为药用，《名医别录》将其列为菜部上品，其“味苦，平，无毒，主安中，利人”，在民间苜蓿主要用于治疗消化不良、肺热咳嗽、黄疸、膀胱结石等症。现代药理作用研究显示，紫花苜蓿黄酮类化学成分具有抗氧化及改善记忆等作用[1-3]。本文采用正交设计法探讨超声提取对新疆紫穗槐总黄酮含量的影响，优选最佳提取工艺，为进一步生产提供科学依据,现报道如下。
1 仪器与试剂
1.1 仪器GBC Cintra 40紫外可见分光光度计(GBC SCIENTIFIC EQUIPMENT PTY.LTD), spectrumlab 22可见分光光度计(上海棱光技术有限公司) ......
(1.新疆医科大学中医学院，新疆 乌鲁木齐 830011，2.北京中医药大学中药学院，北京 100102)
摘 要：目的：研究新疆紫花苜蓿中总黄酮的提取工艺。方法：以总黄酮含量为考察指标，采用正交实验法，考察加醇量、超声提取时间、超声功率、乙醇浓度4个因素对提取工艺的影响。结果：最佳提取工艺为A2B1C1D2，结合生产实际，确定最佳工艺为70%乙醇超声提取20分钟，超声功率为60%，加乙醇量为30倍量。结论：正交实验法优选新疆紫花苜蓿总黄酮的提取工艺简便、快速、准确。
关键词：新疆紫花苜蓿；总黄酮；提取工艺
中图分类号：R284.2文献标识码：A文章编号：1673-7717(2011)03-0646-02
Study on Extracting Flavonoid Compounds from Alfalfa of Xinjiang
LIU Hong-bing1,YAN Xue-hua1,TIAN Shu-ge1,LEI Hai-min2
(1.Xinjiang Medical University,Urumqi 830011,Xinjiang, China;2.Beijing University of Chinese Medicine, Beijing 100102, China)
Abstract:Objective:Study on the extracting flavonoid compounds from Alfalfa of Xinjiang.Methods:With flavonoid content as the inspection indicators, orthogonal test was used to investigate the influence of four extrac-ting factors(ethanol volume, ultrasonic time，ultrasonic power and ethanol consistence)on the extracting process.Results:The optimum extracting process was A2B1C1D2, ie The ethanol volume being 30 times of the weight of medicinal material，extracted for 20 minutes under the power of 60% with ultrasonic extraction,and 70% ethanol consistence.Conclusion:Optimization of extracting process for Alfalfa by orthogonal test is a simple, quick and accuratemethod.
Key words:alfalfa；flavonoid；extracting technology
紫花苜蓿(Medicago sativa L.)为豆科(Leguminosae)苜蓿属(Medicago L.)植物。属重要的饲料植物，素有“牧草之王”的美称，广泛分布于世界各地。紫花苜蓿在我国作为药用，《名医别录》将其列为菜部上品，其“味苦，平，无毒，主安中，利人”，在民间苜蓿主要用于治疗消化不良、肺热咳嗽、黄疸、膀胱结石等症。现代药理作用研究显示，紫花苜蓿黄酮类化学成分具有抗氧化及改善记忆等作用[1-3]。本文采用正交设计法探讨超声提取对新疆紫穗槐总黄酮含量的影响，优选最佳提取工艺，为进一步生产提供科学依据,现报道如下。
1 仪器与试剂
1.1 仪器GBC Cintra 40紫外可见分光光度计(GBC SCIENTIFIC EQUIPMENT PTY.LTD), spectrumlab 22可见分光光度计(上海棱光技术有限公司) ......
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