响应面优化酶法提取平菇多糖工艺研究(1)
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(1.浙江中医药大学,浙江 杭州 310053；2.浙江中医药大学附属第一医院,浙江 杭州 310007)
摘 要：目的:建立平菇多糖的含量测定方法，确定纤维素酶法提取平菇多糖的最佳工艺条件。方法:采用苯酚-硫酸法测定平菇多糖的含量。以平菇多糖得率为指标，考察酶解时间、酶解温度、pH值等3个因素对平菇多糖纤维素酶提取工艺的影响，采用响应面分析法优化工艺条件。结果:平菇多糖的最佳提取工艺条件为酶解时间135min、酶解温度49℃、pH5.30，在此条件下得率可达6.82%。结论 该法成本低、产品得率高，比传统水提法提高3.98%。
关键词：纤维素酶法；平菇；多糖；响应面分析法
中图分类号：R284.2文献标识码：A文章编号：1673-7717(2011)03-0650-04
Optimization of Enzymatic Extraction Technology of Polysaccharides from
Pleurotus ostreatus by Response Surface Methodology
QIAN Lei1,ZHANG Chun-chun1,JIANG Jian-ping1,2,XIONG Yao-kang1
(1.Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China；
2.The Frist Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310007,Zhejiang,China)
Abstract:Objective:To establish determination methods and optimal extraction conditions of polysaccharides from Pleurotus ostreatus treated with cellulase.Methods:Total carbohydrate content was determined by the phenol-sulfuric acid method and the yield of polysaccharides was the indice to determine extraction process by a combination of response surface methodology (RSM).the enzymolysis time,enzymolysis temperature and pH value were selected as factors and a three-factors-three-levels experiment was developed by Box-Benhnken central composite design method.Results:The optimal conditions of variables were determined as follows:enzymolysis time 139 min, enzymolysis temperature 49℃ and pH value 5.3.Under the condition above, the yield of polysaccharides was 6.82%.Conclusion:It was a ideal means with relative low cost and higher extraction efficiency of polysaccharides,which could be improved by 3.98 % through the treatment with cellulase than without.
Key words:cellulase;Pleurotus ostreatus;polysaccharides;response surface methodology
收稿日期：2010-10-08
作者简介：钱磊(1985-) ......
(1.浙江中医药大学,浙江 杭州 310053；2.浙江中医药大学附属第一医院,浙江 杭州 310007)
摘 要：目的:建立平菇多糖的含量测定方法，确定纤维素酶法提取平菇多糖的最佳工艺条件。方法:采用苯酚-硫酸法测定平菇多糖的含量。以平菇多糖得率为指标，考察酶解时间、酶解温度、pH值等3个因素对平菇多糖纤维素酶提取工艺的影响，采用响应面分析法优化工艺条件。结果:平菇多糖的最佳提取工艺条件为酶解时间135min、酶解温度49℃、pH5.30，在此条件下得率可达6.82%。结论 该法成本低、产品得率高，比传统水提法提高3.98%。
关键词：纤维素酶法；平菇；多糖；响应面分析法
中图分类号：R284.2文献标识码：A文章编号：1673-7717(2011)03-0650-04
Optimization of Enzymatic Extraction Technology of Polysaccharides from
Pleurotus ostreatus by Response Surface Methodology
QIAN Lei1,ZHANG Chun-chun1,JIANG Jian-ping1,2,XIONG Yao-kang1
(1.Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China；
2.The Frist Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310007,Zhejiang,China)
Abstract:Objective:To establish determination methods and optimal extraction conditions of polysaccharides from Pleurotus ostreatus treated with cellulase.Methods:Total carbohydrate content was determined by the phenol-sulfuric acid method and the yield of polysaccharides was the indice to determine extraction process by a combination of response surface methodology (RSM).the enzymolysis time,enzymolysis temperature and pH value were selected as factors and a three-factors-three-levels experiment was developed by Box-Benhnken central composite design method.Results:The optimal conditions of variables were determined as follows:enzymolysis time 139 min, enzymolysis temperature 49℃ and pH value 5.3.Under the condition above, the yield of polysaccharides was 6.82%.Conclusion:It was a ideal means with relative low cost and higher extraction efficiency of polysaccharides,which could be improved by 3.98 % through the treatment with cellulase than without.
Key words:cellulase;Pleurotus ostreatus;polysaccharides;response surface methodology
收稿日期：2010-10-08
作者简介：钱磊(1985-) ......
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