冠心合剂对家兔颈动脉球囊损伤后Ⅰ型胶原表达的影响(1)
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摘 要：目的：观察冠心合剂对兔颈动脉经皮冠状动脉腔内成形术(PTCA)损伤后细胞外基质Ⅰ型胶原蛋白表达的影响。方法：运用球囊原位扩张、拉伤致兔颈总动脉损伤建立PTCA术后内皮损伤模型。从术前3天开始各治疗组予相应剂量冠心合剂灌胃给药，分别于2、4、6周处死动物，免疫组织化学法检测I型胶原的表达。结果：各中药治疗组内膜胶原密度较模型组降低，差异有统计学意义(P<0.05)；冠心合剂大剂量组6周的内膜胶原密度表达最少。结论：冠心合剂可保护兔颈动脉球囊损伤后内膜，其机制可能与减少血管壁胶原含量有关。
关键词：冠心合剂；球囊损伤； I型胶原
中图分类号：R540 文献标识码：A 文章编号：1673-7717(2011)04-0689-03
Effect of Guanxin Decoction on Expression of Type-I collagen
in Rabbits after Carotid Artery Balloon Injury
ZHU Guang-li, ZHOU Fan, FANG Wei, WEI Li-ping, LIU Zhao
(Department of Cardiology, Guangxing Hospital Affiliated Zhejiang Chinese
Medicine University, Hangzhou 310007，Zhejiang, China)
Abstract:Objective：To observe the effect of Guanxin decoction on extracellular matrix collagen Ⅰexpression after percutaneous transluminal coronary angioplasty (PTCA) of rabbits. Methods：Endothelial injury models were established by using in-situ balloon expansion and strain-induced injury of rabbit carotid artery. The Guanxin decoction groups were fed correspondingly does 3 days before the operation. All the animals were sacrificed at the end of the 2ed, 4th and 6th weeks respectively and operated carotid arteries were obtained. Immunohistochemical Strept Avidin-Biotin complex (SABC) staining were used for observing arterial morphology and intimal collagen density. Results：The Guanxin decoction groups have lower levels of intimal hyperplasia and intimal collagen (P<0.05). The large-does Guanxin decoction group has the lowest densities of intimal collagen at the end of the 6th week. Conclusion：Guanxin decoction could decreased the neointimal hyperplasia after PTCA , the mechanism may relate to inhibiting the expressions of collagen typeⅠin the injured artery.
Key words:Guanxin Decoction Balloon Injury Type-I collagen
经皮腔内冠状动脉成形术(PTCA) 已成为冠心病心肌血管再通的重要手段，但术后再狭窄已成为影响其疗效的主要因素。血管组织中胶原增生是引起病变的主要因素，其参与了再狭窄的形成过程【sup】[1]【/sup】。本研究通过建立在体兔颈动脉球囊损伤模型，采用免疫组织化学方法，观察冠心合剂对实验性动脉球囊损伤后Ⅰ型胶原蛋白表达的影响。
1 实验材料与方法
1.1 实验动物
健康纯种雄性新西兰大白兔92只, 体重1.8～2.3kg，由浙江中医药大学动物中心提供。
1.2 主要实验药品及试剂
冠心合剂(黄芪30g，蒲黄20g，五灵脂20g)，将各药加水煎煮2次，第1次1.5h，第2次0.5h，浓缩成2g/mL的生药煎剂(杭州市中医院制剂室制备)。戊巴比妥钠(国药集团化学试剂有限公司)；青霉素钠(华北制药有限公司)；SABC一抗、山羊抗兔IgG抗体-HRP多聚体(武汉博士德公司) ......
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