运动区久留针对局灶性脑缺血大鼠血管生成素-1表达的影响(1)
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摘 要：目的：观察运动区久留针对局灶性脑缺血模型大鼠脑组织中血管生成素-1(Ang-1)表达的影响。方法：随机选取66只Wistar雄性大鼠采用改良线栓法建立局灶性脑缺血大鼠模型，43只大鼠复制成功模型后随机分为假手术组(14只)、模型组(14只)、久留针组(14只)、短留针组(15只)，并设假手术组(14只)进行对照研究，采用改良线栓法建立局灶性脑缺血大鼠模型，选取大鼠运动区进行针刺留针治疗，久留针组留针10h，短留针组留针30min，每天1次，治疗2周，模型组及假手术组不作针刺治疗。对各组大鼠的神经功能进行评分，并用免疫组化法检测血管生成素(Ang-1)的表达。结果：与模型组比较，久留针组的Zealonga评分及平衡木行走试验评分减少(P < 0.05)，Ang-1表达升高(P<0.05)。结论：运动区久留针能改善局灶性脑缺血大鼠的运动功能，促进梗塞区Ang-1的表达。
关键词：头皮针；运动区；Ang-1；局灶性脑缺血；Wistar大鼠
中图分类号：R285.5 文献标识码：A 文章编号：1673-7717(2012)01-0079-03
Effect of Long Time Retention of Needle on Angiogenin-1 Expression of Motor-area in Brain Tissue of Rat with Focal Cerebral Ischemia
BAO Yehua, ZENG Youhua,WANG Yanwu, WANG Chongmin, CHU Jiamei
(Hangzhou Hospital of TCM, Hangzhou 310007, Zhejiang, China)
Abstract:Objective: To observe the effect of long time return needle on motor area on angiogenin-1 in the brain tissue of the rat after focal cerebral ischemia reperfusion. Methods: 66 Male Wistar rats were used to prepare the focal cerebral ischemia reperfusion model by filment occlusion. After the model had been made, 43 rats were randomly divided into four groups: sham operation group,Model group (n=14), Long time return needle group(n=14), Short time return needle group(n=15). Sham Operation Group (n=14) was arranged at the same time. A Wistar rat model of focal cerebral ischemia reperfusion was made by filament occlusion. Motor area was selected as the treatment point. Long-return Needle group was returned the needle for 10 hours and shortShort-return needle group for 30 minute per day for 2 weeks. No special treatment was used on the rats of the other groups. The neurological scores were observed before and after treatment. Angiopoietin- 1 was detected with by immunohistochemical method. Result: After 2 weeks, the neurological scores of long-return needle group were higher compared with model group(P<0.05) . Angiopoietin- 1 was up- regulated in long time return needle group (P<0.05) . Conclusion: Long time return needle on motor area can improve the movement function of the rat after focal brain ischemia, which may be associated with the up- regulation of Angiopoietin-1 ......
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