软脉煎对实验性晚期动脉粥样硬化家兔平滑肌细胞凋亡的调控作用(1)
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摘 要：目的：通过对颈动脉粥样硬化家兔模型的治疗观察,研究软脉煎对动脉粥样硬化形成晚期血管平滑肌细胞Bcl-2、Bax及Caspase-8表达的影响。方法：采用高脂喂养加颈动脉内膜空气干燥术制备家兔颈动脉粥样硬化模型，38只新西兰家兔随机分成4组，正常对照组8只，模型组8只，软脉煎组11只，辛伐他汀组11只，12周后采用免疫组织化学法检测Bcl-2、Bax、Caspase-8的表达。结果：软脉煎组与模型组比较，能升高Bcl-2蛋白水平，降低Bax、Caspase-8蛋白表达，从而升高Bcl-2/Bax比值，具有显著性意义(P<0.05)。结论：软脉煎能调控动脉粥样硬化形成晚期平滑肌细胞凋亡信号途径中Bcl-2、Bax及Caspase-8的表达，从而提高斑块的稳定性，减少心脑血管事件的发生。
关键词：软脉煎；动脉粥样硬化；细胞凋亡；Bcl-2；Bax；Caspase-8
中图分类号：R285.5 文献标识码：A 文章编号：1673-7717(2012)01-0093-03
Control Effects of Ruanmai Decoction on Apoptosis of Rabbit's Smooth Muscle Cell
in Experimental Advanced Atherosclerosis
PAN Luqian,GU Yun
(Longhua Hospital Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China)
Abstract:Objective：To explore the effects of Ruanmai Decoction on expressing Bcl-2, Bax and Caspase-8 of vascular smooth muscle cell in experimental advanced atherosclerosis by observing the carotid atherosclerotic rabbit models.Methods：The carotid atherosclerotic rabbit models were induced by high-cholesterol-diet plus air-drying. Thirty-eight New Zealand rabbits were randomly divided into four groups, the normal control group (n=8), the model group (n=8), the Ruanmai Decoction group (n=8) and the simvastatin group (n=11). The expressive level of Bcl-2, Bax and Caspase-8 were assayed by the method of immunohistochemistry after twelve-week treatment.Results：Compared with the model group, the treatment of Ruanmai Decoction could raise the levels of Bcl-2 protein but reduce the level of Bax and Caspase-8 protein, therefore the Bcl-2/Bax ratio increased. There's significant difference among groups (P<0.05).Conclusion：Ruanmai Decoction could control the expression of Bcl-2, Bax and Caspase-8 of vascular smooth muscle cell in advanced atherosclerosis, which could enhance the stability of atherosclerotic plaque and reduce the incidence rate of cardiovascular and cerebrovascular disease.
Key words:Ruanmai Decoction; Apoptosis; Atherosclerosis; Bcl-2；Bax；Caspase-8
动脉粥样硬化(atherosclerosis，AS)是临床常见病、多发病，是冠心病、脑血管意外等疾病的共同病理基础，严重威胁人类的身体健康，是人类死亡的第一号杀手[1]，防治AS成为医学界共同关注的重要课题。AS的病因和发病机理十分复杂，近年来细胞凋亡在AS形成和发展中的作用逐渐成为研究的热点。中医典籍中无AS的病名，常将其归属为痰证、瘀证、脉痹等范畴[2]，治疗上也多从活血化瘀、健脾化痰等方法着手。而本研究采用具有补肾益气作用的中药复方软脉煎治疗AS模型家兔 ......
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