模拟中医病因所致脾气虚、脾阳虚大鼠模型的证候比较研究(1)
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摘 要：目的：以多因素复合法分别建立脾气虚证、脾阳虚证大鼠模型，对两组模型大鼠进行比较研究以探讨脾气虚、脾阳虚的证候实质与差异。方法：动物随机分为正常组、脾气虚组和脾阳虚组，以相应造模方法进行模型复制，并以四君子汤和理中汤进行药物反证。结果：脾气虚和脾阳虚大鼠宏观证候计分高于正常组(P<0.01)，且以脾阳虚组为著(P<0.01)；两组血清GAS均低于正常组(P<0.01)；脾气虚组的血浆MTL低于正常组(P<0.01)，而脾阳虚组高于正常组(P<0.01)；经健脾中药干预后，各项脾虚症状和体征逐渐缓解或消失；血清GAS、血浆MTL紊乱回复。结论：多因素复合法复制的脾气虚、脾阳虚证大鼠模型符合证候的生物学特征，稳定性良好，且经得起药物反证；脾气虚、脾阳虚大鼠的脾虚宏观证候呈递进关系；血浆MTL在脾气虚和脾阳虚中变化趋势不一致，可作为鉴别两证的微观指标。
关键词：多因素；脾气虚证；脾阳虚证；大鼠模型；血清GAS；血浆MTL
中图分类号：R285.5 文献标识码：A 文章编号：1673-7717(2012)01-0104-04
Comparative Study on Model Rats with Spleen-qi Deficiency Syndrome and Spleen-yang Deficiency Syndrome
WANG Ying, ZHENG Xiaowei, LI Xiuyue
(Zhejiang Chinese Medical University,Hangzho 310053,Zhejiang,China)
Abstract:Objective: By making a comparative study on rats with spleen-qi deficiency syndrome and spleen-yang deficiency syndrome, to seek the objective property of these two syndromes and their differences. Methods: Animals were randomly divided into three groups in terms of control group, group of spleen-qi deficiency syndrome and spleen-yang deficiency syndrome. Model rats were induced by combination of multi-methods and testified with corresponding medicinals (with Sijunzi Decoction for spleen-qi deficiency syndrome and Lizhong Decoction for spleen-yang deficiency syndrome). Results: Scores of symptoms and signs in two model groups were significantly higher than those in control group (P<0 01), and the difference between two model groups was significant as well, with group of spleen-yang deficiency higher (P<0 01). Serum GAS was significantly decreased in model groups(P<0 01). Plasma MTL was significantly decreased in group of spleen-qi deficiency (P<0 01), while increased in spleen-yang deficiency (P<0 01). After being treated with medicinals, the indexes mentioned above were recovered somehow. Conclusion: Multi-methods applied in this research to induce spleen-qi deficiency syndrome and spleen-yang deficiency syndrome are proved to be feasible and can be popularized. There is a calculable progressing in symptoms and signs between spleen-yang deficiency and spleen-qi deficiency. The variation tendency of plasma MTL in spleen-qi deficiency and spleen-yang deficiency is inconsistent, which may be regarded as an objective index to make syndrome differentiation ......
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