黄芪建中汤对脾气虚证肺癌转移小鼠TIMP1基因转录的影响(1)
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摘 要：目的：观察黄芪建中汤对脾气虚证肺癌转移小鼠TIMP1基因转录的影响。方法：用苦寒泻下法建立脾气虚证Lewis肺癌转移小鼠模型，药物治疗组小鼠分别给予不同的药物处理，用药15天后，眼球取血，脱颈椎处死小鼠，无菌剥取瘤块，取肺及肿瘤组织。检测血清木糖及胃泌素水平，计算抗转移率，采用RT-PCR方法检测TIMP1-mRNA转录水平。结果：黄芪建中汤低、中、高剂量组均可抑制转移灶，上调TIMP1-mRNA的转录水平，以高剂量组明显，与模型组比较有统计学意义。结论：黄芪建中汤上调TIMP1-mRNA的转录，可能是其抑制肿瘤转移的机理之一。
关键词：黄芪建中汤；脾气虚；肺癌；TIMP1-mRNA
中图分类号：R285.5
文献标识码：A
文章编号：1673-7717(2012)04-0686-03
Influence of Huangqi Jianzhong Decoction on TIMP1 Transcribe of Mice
With Lung Cancer Metastasis and Spleen-Qi Deficiency Syndrome
BAO Su-zhen1，ZHENG Xiao-wei1，SONG Hong1，CAO Yue-jiao1，SUN Zai-dian2
(1.Zhejiang Chinese Medical University，Hangzhou 310053，Zhejiang，China；
2.Zhejiang Provincial Hospital of Traditional Chinese Medicine，Hangzhou 310006，Zhejiang，China)
Abstract:Objective：The influence of Huangqi Jianzhong Decoction on TIMP1 transcribe of mice with lung cancer metastasis and spleen-Qi deficiency syndrome. Methods：The Lewis mice model with spleen-Qi deficiency was established by purging with cold and bitter drugs. Treatment groups were given different medicine respectively. 15 days after treatment， mice were killed to get blood from eyeballs. The tumor tissue and lung were taken out. Levels of blood xylose and gastrin were measured；the anti-transfer ration was counted；TIMP1-mRNA transcribe was tested by RT-PCR.Results：The low，medium and high dose of Huangqi Jianzhong Decoction groups all can anti neoplasm metastasis and increase the level of TIMP1-mRNA transcribe. And the high dose group was obvious，and had significant difference compared with model group. Conclusion：Huangqi Jianzhong Decoction can increase TIMP1-mRNA transcribe，which may be one of the anti neoplasm metastasis mechanisms.
Key words:Huangqi Jianzhong Decoction；spleen-Qi deficiency；lung cancer；TIMP1-mRNA
恶性肿瘤的侵袭和转移是一个多阶段、多步骤进行的一个极其复杂的过程，在此过程中，肿瘤对组织细胞外基质和基底膜的降解是肿瘤细胞能否形成转移的关键步骤[1]。金属蛋白酶(MMPS)能降解细胞外基质的成分，促进肿瘤的扩散和转移。金属蛋白酶抑制剂(TIMPS)是MMP的特异性抑制剂，在调控MMPS的活性方面起着重要作用。TIMPS参与了人体多种生理病理过程,涉及多种细胞因子及ECM间的调节关系。因此，TIMPs在肿瘤组织或血清中表达及分泌水平的检测，对于恶性肿瘤的诊断以及预后估计有重要价值。本实验旨在探讨脾气虚证对肺癌小鼠的TIMP1-mRNA转录的影响及黄芪建中汤干预作用的可能机制。
1 实验材料
1.1动物及瘤株
C57BL/6小鼠100只，雌性，体重(20±2)g，购于上海医学科学院实验动物中心。小鼠Lewis肺癌瘤株，购于上海医学科学院实验动物中心。
1.2药物
黄芪建中汤：黄芪9g，芍药18g，桂枝9g，炙甘草6g，生姜10g，大枣4枚，饴糖30g ......
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