榄香烯对人胃癌细胞SGC-7901的体外生长及CD44v6、CD44的影响(1)
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摘 要：目的:探讨榄香烯对人胃癌细胞株SGC-7901体外生长及细胞黏附分子CD44v6、CD44的影响。方法:Cell Counting Kit-8(CCK-8)检测榄香烯对SGC-7901细胞增殖的影响；采用DAPI荧光染色法进行细胞核形态学分析；流式细胞仪检测细胞膜表面CD44v6、CD44表达。结果: 榄香烯抑制SGC–7901细胞增殖，其呈量效和时效关系；DAPI 染色显示榄香烯诱导SGC-7901 细胞凋亡并显示特征性的凋亡细胞核形态；流式细胞仪检测结果显示榄香烯20、30、40μg/mL组与对照组相比，SGC-7901细胞膜表面CD44v6表达明显减少(P<0.01)，CD44表达的差异无统计学意义(P>0.05)。结论:榄香烯能抑制SGC-7901细胞增殖、诱导细胞凋亡，其抗肿瘤转移的机制可能与下调CD44v6表达相关。
关键词：榄香烯；人胃癌细胞株, SGC-7901；CD44v6；抗转移
中图分类号：R285.5
文献标识码：A
文章编号：1673-7717(2012)04-0736-03
Effects of elemene on CD44v6,CD44 and the proliferation of human gastric carcinoma cell line SGC-7901
YE Ya-jia1，ZHANG Sheng-hui2，WANG Hai-bo1，LANG Wei1
(1.Department of Traditional Chinese Medicine, Wenzhou 325000 , Zhejiang , China;
2.Labarotary of internal medicine, the First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000 , Zhejiang , China)
Abstract:Objective: To investigate the effects of elemene on the proliferation and cell adhesion molecule CD44v6、CD44 changes of human gastric carcinoma cell line SGC-7901. Methods: The proliferation of SGC-7901 cells was measured by CCK-8 assay. Nuclear morphology was observed with DAPI staining. The expression levels of CD44 v6 and CD44 were analyzed using flow cytometry. Results: Elemene inhibited the proliferation of SGC-7901 cells in a dose-and time-dependent manner. After treatment with elemene, the apoptosis of SGC-7901 cells was induced and the characteristic apoptosis nuclei were observed with DAPI staining. Compared with the control group, the expression of CD44v6 in SGC-7901 cells treated by 20、30、40 μg/mL element decreased significantly after 48h (P<0.01),but there was no statistical significance in the difference of CD44 expression.Conclusion: Elemene has a strong anti-tumor activity to SGC-7901 cells, and its function of anti-metastasis may be related to the reduction of CD44v6 expression.
Key words:Elemene; human gastric carcinoma cell SGC-7901; CD44v6; anti-metastasis
榄香烯(elemene)是从姜科植物温郁金(温莪术 ......
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