电针督脉和膀胱经对实验性抑郁症大鼠行为学及海马NSE表达的影响(1)
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摘 要：目的：观察电针督脉经穴(百会、神庭)加膀胱经穴(心俞、肝俞)对实验性抑郁症大鼠行为学和海马NSE阳性细胞表达数量的影响。方法：采用慢性应激结合孤笼饲养制作大鼠抑郁症模型，用旷场法进行行为学测定以及计算大鼠糖水消耗量，并运用免疫荧光双标技术观察对抑郁症大鼠海马NSE阳性细胞表达数量的影响。结果：电针督脉加膀胱经穴可显著改善抑郁症大鼠的体重增加迟缓，糖水消耗增多，行为学评分升高，使抑郁症大鼠海马CA1、CA3区及齿状回(DG)神经元数目显著增加。结论：针刺督脉加膀胱经穴可以使抑郁大鼠活动增多、快感增加、体重增加,减轻海马神经元的损伤，从而促进大鼠抑郁的改善。
关键词：电针；督脉；膀胱经；抑郁症；行为；NSE
中图分类号：R245文献标识码：A
文章编号：1673-7717(2012)04-0833-03
Effects of Du Meridian and Foot Taiyang Meridian Electro-acupuncture on Behavior
Changes and Expression of NSE on Hippocampal in Depression Rats
ZHUO Yuan-yuan,PI Min
(The Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Shenzhen 518033,Guangdong，China)
Abstract:Objective: To study the effects of Du meridian and foot Taiyang meridian eletro-acupuncture on behavior changes and expression of NSE on hippocampal in the treatment of depression.Methods:The depression models of rats were established by chronic unpredictable mild stress combining raising seperately. The rearing score and crossing score of rats were measured by open-field test and sweet water wastage was detected and the numbers of NSE positive cells were observed by double-signed immunofluorescence.Results:Du meridian and foot Taiyang meridian eletro-acupuncture could put on the pounds and increase sweet water wastage, improve the locomotor behavior of the depression rats and increase the numbers of nerve cells in CA1,CA3 and dentate gyrus on hippocampal.Conclusion:Du meridian and foot Taiyang meridian eletro-acupuncture can improve the depression rats' activity, pleasant feeling, body weight and protect nervel cells in hippocampal to treat depression.
Key words:eletro-acupunture; Du meridian; Foot Taiyang meridian; depressive disorder; behavior; NSE
抑郁症是一种以情绪低落、思维缓慢以及言语动作减少、迟缓为主要特点的情感障碍性精神疾病。目前比较一致的观点认为抑郁症的发生与神经元再生障碍密切相关[1-2]。研究证明，神经元再生在成年哺乳动物海马脑区的减少和增多分别是导致抑郁症发生和恢复的重要因素[3]。因此，促进神经元再生可能是抗抑郁治疗阻止或逆转由应激所导致的海马神经元萎缩和丢失的机制之一。笔者根据“脑与五脏六腑相互为用”的特点，结合督脉、膀胱经与脑的密切关系，观察电针督脉百会、神庭和足太阳膀胱经的心俞、肝俞对抑郁模型大鼠行为学的影响以及海马神经元再生的作用。
1 材料和方法
1.1 实验动物 健康Wistar大鼠54只，雌雄各半，体重200～220g。整个实验过程中动物自由摄食和饮水(术前禁食，禁食禁水应激除外)，大鼠饲料为广东省实验动物中心提供的富含多种成分的配方。饲养环境：深圳市中医院中心实验室提供的动物实验房，室温18℃～25℃(热、冷应激除外)，相对湿度60%～70%,光照12h∶12h(光∶暗)。实验室：深圳市中医院临床细胞分子生物学三级国家实验室 ......
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