RV-HSV-TK/GCV自杀基因系统治疗宫颈癌的实验研究
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[ Abstract] Objective To study in vitro and in vivo killing effect and the bystander effect of HSV-TK/GCV suicide gene systemon cervix cancer cell line Hula. Methods GINaTK letroviral vector containing HSV-TK gene was transduced into PA317 packaging cellby lipofectin. Cervix cancer cell line Hela was infected by high titer viral supematant. HelsdTK cells and Hela cells were used in in vitroand in vivo study. In in vitro study, sensitivity of Hela/TK cells to GCV and bystander effect were observed by MTT test. In in vivostudy, Hela/TK cells end Hela cells were injected s.c. into flanking BALB/C mice,GCV was administraated i.p. ,RT-PCR was appliedto detect the expression of HSV-TK gene. Statistical analysis of the data was performed by using the ANOVA(analysis of variance). Re-suits The Hela cells transfered with GINaTK gene displayed a higher antitumor effect than parent cells. In in vitro study, when the ra-rio of HelaJTK cells reached 10 %, the tumor cell-killing proportion was 50%. In in viw study, all mice developed tumors in 7 days af-ter tumor cells implanted, the ratio of tumors formation was 100%. GCV could suppress tumor fomation of the Hula/TK cells. After micetreated with CCV, the median tumor volume of mice implanted vdth Hela/TK cells and mice with cells mixed was decreased to 52.8 %( P
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