背向散射积分评价自发性高血压大鼠左室心肌纤维化及苯那普利的干预作用(1)
[摘要] 目的 通过分析超声背向散射积分(IBS)参数的变化评价苯那普利对自发性高血压大鼠(SHR)左室心肌纤维化的影响。方法 14只雄性SHR随机分成模型对照组(SHR组，n=7)和药物干预组(SB组,n=7),另外选择7只雄性Wistar京都大鼠(WKY)作为正常对照组(WKY组)。SB组给予苯那普利灌胃(10 mg•kg-1•d-1)，WKY及SHR组给予等量蒸馏水灌胃。每周秤体质量，调节用药量。12周后超声测量左室射血分数(LVEF)、IBS参数(包括IBS%和CVIB)；对心肌组织进行Masson染色、嗜银染色及结缔组织生长因子(CTGF)免疫组织化学染色。结果 3组间比较IBS%值SHR组>SB组(PWKY组(PSB组>WKY组(均P<0.01)。IBS%与CTGF、CVF、PVCA、APFVF、T/D呈正相关。结论 高血压大鼠存在明显心肌纤维化，心肌CTGF表达增加；苯那普利能抑制CTGF的表达，在一定程度上逆转心肌间质纤维化，改善左室收缩功能。超声IBS技术可用于评价这种变化。
, 百拇医药 [关键词] 声学密度定量技术；背向散射积分；苯那普利；自发性高血压大鼠；心肌纤维化；结缔组织生长因子；
[中图分类号] R-33；R445.1[文献标识码] A[文章编号] 1671-7562(2008)03-0245-05
Study of the left ventricular myocardial interstitial fibrosis and effect of benazepril
in spontaneous hypertension rats by integrated backscatter technique
XUE Rui-feng1,SUN Shao-xian1, SUN Dong-mei1,SONG Wen-jing1，ZHANG Shu-hua2,XIE Fang2
, 百拇医药
(1.Department of Post-graduate,North China Coal Medical University， Tangshan 063000,China；2.Department of
Ultrasound Diagnosis,Afiliated Hospital of North China Coal Medical University，Tangshan 063000,China)
Abstract: Objective To assess left ventricular myocardial interstitial fibrosisand effects of benazepril on spontaneous hypertension rat(SHR)by integrated backscatter(IBS)technique. Methods Fourteen SHRs were divided equally into two groups: intervening subject(SB)group and control subject(SHR)group. Other 7 WKYs were as the normal subjects(WKY).SB group were given 10 mg•kg-1•d-1 body weight of benazepril by intragastric administrstion,and WKY group and SHR group received 10 mg•kg-1•d-1 of distilled water. The animals were weighed once week to adjust for drug dosage. After 12 weeks, IBS%, CVIB, left ventricular ejection(LVEF)were determined.Masson staining and argyrophilic staining were used in the study of collagen volume fraction(CVF),perivascular collagen area(PVCA)，ratio of small coronary artery wall thickness to external diameter(T/D)and argyrophilic fibers volume fraction(APFVF)in heart tissue. The expression of CTGF was evaluated by qualitative and semiquantitative immunohistochemical staining. ResultsIBS% was greater in SHR group than SB group(P<0.01)and SB group greater than WKY group(P<0.05),while CVIB was lower in SHR group than SB groupand SB group less than WKY group(P<0.05). CTGF,CVF,PVCA,T/D,APFVF were increased in SHR group than SB group, while SB group greater than WKY group(all P<0.01). The correlations between IBS% and CTGF,CVF，PVCA， APFVF,T/D were significant. ConclusionsThe myocardial interstitial fibrosis in SHR is obvious. Hypertension induced the upregulation of CTGF in myocardium of rat. Benazepril can decrease the expression of CTGF and has good a effect on left ventricular myocardial interstitial fibrosis which contributes to reserve left ventricular systolic function.It is feasible to assess this change by IBS technique.
Key words： ultrasonic；integrated backscatter；benazepril；spontaneous hypertension rats；myocardial interstitial fibrosis；connective tissue growth factor, http://www.100md.com(薛瑞凤 孙绍贤 孙冬梅 宋文静 张树华 谢 芳)
, 百拇医药 [关键词] 声学密度定量技术；背向散射积分；苯那普利；自发性高血压大鼠；心肌纤维化；结缔组织生长因子；
[中图分类号] R-33；R445.1[文献标识码] A[文章编号] 1671-7562(2008)03-0245-05
Study of the left ventricular myocardial interstitial fibrosis and effect of benazepril
in spontaneous hypertension rats by integrated backscatter technique
XUE Rui-feng1,SUN Shao-xian1, SUN Dong-mei1,SONG Wen-jing1，ZHANG Shu-hua2,XIE Fang2
, 百拇医药
(1.Department of Post-graduate,North China Coal Medical University， Tangshan 063000,China；2.Department of
Ultrasound Diagnosis,Afiliated Hospital of North China Coal Medical University，Tangshan 063000,China)
Abstract: Objective To assess left ventricular myocardial interstitial fibrosisand effects of benazepril on spontaneous hypertension rat(SHR)by integrated backscatter(IBS)technique. Methods Fourteen SHRs were divided equally into two groups: intervening subject(SB)group and control subject(SHR)group. Other 7 WKYs were as the normal subjects(WKY).SB group were given 10 mg•kg-1•d-1 body weight of benazepril by intragastric administrstion,and WKY group and SHR group received 10 mg•kg-1•d-1 of distilled water. The animals were weighed once week to adjust for drug dosage. After 12 weeks, IBS%, CVIB, left ventricular ejection(LVEF)were determined.Masson staining and argyrophilic staining were used in the study of collagen volume fraction(CVF),perivascular collagen area(PVCA)，ratio of small coronary artery wall thickness to external diameter(T/D)and argyrophilic fibers volume fraction(APFVF)in heart tissue. The expression of CTGF was evaluated by qualitative and semiquantitative immunohistochemical staining. ResultsIBS% was greater in SHR group than SB group(P<0.01)and SB group greater than WKY group(P<0.05),while CVIB was lower in SHR group than SB groupand SB group less than WKY group(P<0.05). CTGF,CVF,PVCA,T/D,APFVF were increased in SHR group than SB group, while SB group greater than WKY group(all P<0.01). The correlations between IBS% and CTGF,CVF，PVCA， APFVF,T/D were significant. ConclusionsThe myocardial interstitial fibrosis in SHR is obvious. Hypertension induced the upregulation of CTGF in myocardium of rat. Benazepril can decrease the expression of CTGF and has good a effect on left ventricular myocardial interstitial fibrosis which contributes to reserve left ventricular systolic function.It is feasible to assess this change by IBS technique.
Key words： ultrasonic；integrated backscatter；benazepril；spontaneous hypertension rats；myocardial interstitial fibrosis；connective tissue growth factor, http://www.100md.com(薛瑞凤 孙绍贤 孙冬梅 宋文静 张树华 谢 芳)