应用背向散射积分技术评价糖尿病大鼠心肌纤维化(1)
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[摘要]目的 检测不同病程糖尿病模型大鼠的心肌背向散射积分(IBS),同时观察纤维化指标,探讨IBS与心肌纤维化的关系。方法 腹腔注射链尿佐菌素(65 mg•kg-1)建立SD大鼠糖尿病模型,于第4、12、24周测定常规心功能参数(LVEF)、左室后壁校正IBS值(IBS%)、IBS周期性变异幅度(CVIB)。同期检测心肌纤维化指标:心肌羟脯氨酸含量(HPC),Masson染色观察胶原纤维容积分数(CVF)及血管周围胶原面积(PVCA),用免疫组织化学法对心肌胶原蛋白在心肌的分布及表达进行半定量分析,光镜与透射电镜对心肌行组织学观察,并与同龄正常大鼠进行比较。结果 (1)与对照组比较,糖尿病组的IB%明显增高(P<0.05),CVIB第4、12周时尚无明显变化(P>0.05),第24周时明显降低(P0.05);糖尿病组心肌纤维化指标HPC、CVF、PVCA、胶原蛋白Ⅰ和Ⅲ的表达均明显增高(P<0.05)。(2)IBS%与HPC、CVF、PVCA、胶原蛋白Ⅰ和Ⅲ表达水平呈正相关(P<0.05)。(3)电镜发现糖尿病组心肌细胞肌丝稀疏,线粒体肿胀,间质胶原增生。结论 胶原蛋白Ⅰ持续性增加是糖尿病模型鼠心肌纤维化的主要原因。通过监测IBS可以观察糖尿病大鼠心肌组织纤维化的程度。
[关键词] 超声描记术;背向散射积分;糖尿病大鼠;心肌纤维化
[中图分类号] R363.2
[文献标识码] A
[文章编号]1671-7562(2009) 04-0261-06
Study on myocardial fibrosis in diabetes mellitus using ultrasonic integrated backscatter parameters
GUO Shu-xiang,SUN Dong-mei,XUE Rui-feng
(Department of Post-graduate,North China Coal Medical University,Tangshan 063000,China)
Abstract:Objective To investigate the relation between myocardial ultrasonic integrated backscatter(IBS) and myocardial fibrosis of diabetic rats in the different stages.Methods The diabetes mellitus in healthy male Sprague-Dawley rats was induced by a single injection of
streptozotocin(STZ,Sigma) into intraperitoneal at a dose of 65 mg•kg-1 body weight.Left ventricular ejection fraction(LVEF),myocardial calibrated IBS(IBS%),the cyclic variation of IBS(CVIB) by IBS,were determined in the rats of 4,12 and 24 weeks after DM was induced.The myocardial hydroxyproline concentration(HPC) was performed.Masson’ trichrome staining were used in the study of total collagen area.The type Ⅰ and Ⅲ collagen protein expression was evaluated in heart by qualitative and semiquantitative immunohistochemical staining,while the age-matched normal rats served as control group.Myocardiopathological change were observed with light microscope and transmission electron microscope.Results (1)The IBS% of left ventricular posterior wall was significantly higher in diabetic rats than those in the control group(P<0 ......
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