不同时间血液灌流对脓毒症兔血IL-6及TNF-α水平的影响(1)
 |
| 第1页 |
参见附件(2891KB，5页)。
[摘要] 目的:通过动态检测脓毒症兔血IL-6及TNF-α水平变化,探讨行血液灌流最佳时机。方法：将18只新西兰大白兔随机分成6组,通过盲肠结扎穿孔法制作脓毒症模型,分别在术后3、6、12、24、48 h行单次血液灌流,同时设立空灌流组(对照组),并均在手术前，术后3、6、9、12、24、48 h动态检测血清中IL-6及TNF-α水平,记录各组兔子的生存时间。结果：术后12 h行血液灌流组血IL-6平均水平最低(854.2 pg•ml-1),生存时间最长(73.3 h),P<0.05；而术后3 h行血液灌流组血TNF-α平均最低(68.2 pg•ml-1,P<0.05),并使TNF-α峰值降低,但生存时间较对照组未见延长。结论：血液灌流在脓毒症早中期进行,能有效降低血前炎症细胞因子TNF-α及IL-6水平,并使实验兔生存时间延长。
[关键词] 血液灌流； 脓毒症； 白介素6； 肿瘤坏死因子α
[中图分类号] R631； R392.11 [文献标识码] A [文章编号] 1671-7256(2011)02-0129-05
doi：10.3969/j.issn.1671-7256.2011.02.003
Effect of hemoperfusion in different time on blood IL-6
and TNF-α of sepsis rabbits
JI Guo-ye, WANG Bin, DU Jiang, ZHANG Jian
(Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China)
[Abstract] Objective: To investigate the best timing to perform hemoperfusion for sepsis rabbits by detecting the dynamic concentrations of IL-6 and TNF-α. Methods: 18 rabbits were randomly divided into 6 groups. They were treated with hemoperfusion once respectively in 3 hours, 6 hours, 12 hours, 24 hours, 48 hours after the cecal ligature and puncture surgery, control group underwent fake perfusion. The blood concentration of IL-6 and TNF-α were dynamically detected before the surgery respectively, after 3 hours, 6 hours, 9 hours, 12 hours, 24 hours, 48 hours. Then we noted down the rabbit survival time. Results: The average concentration of blood IL-6 in 12 hours hemoperfusion group was the lowest(854.2 pg•ml-1), accompanying the longest survival time(P<0.05); Nevertheless, the average concentration of blood TNF-α in 3 hours hemoperfusion group was the lowest(68.2 pg•ml-1), P<0.05, accompanying the declining of the peak, but not prolonging the survival time. Conclusion: Hemoperfusion in the early and medium-term progress of sepsis can effectively reduce the blood concentration of pro-inflammatory cytokines TNF-α and IL-6, and the later one can make the experiment rabbits survival a longer time, instructing the decision of our clinical hemoperfusion timing.
[Key words] hemoperfusion； sepsis； IL-6； TNF-α ......
您现在查看是摘要介绍页，详见PDF附件(2891KB，5页)。