大鼠全身性缺血-再灌注损伤模型的建立(1)
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[摘要] 目的:建立大鼠全身性缺血-再灌注损伤模型。方法:30只大鼠快速放血至平均动脉压(MAP)40 mmHg,慢速放血维持20 min,室温放置1 h,全部失血回输。3 h后将大鼠处死,期间监测大鼠MAP、心率、肛温、心电图,并检测大鼠放血前及再灌注后3 h血浆天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、乳酸脱氢酶(LDH)值。取心、肾、脑、肺标本作组织病理检测。结果:放血结束时及再灌注前MAP、心率、肛温符合正态分布。再灌注后3 h血浆AST、ALT、LDH活性均显著高于放血前(P<0.01)。大鼠失血后心电图发生缺血样改变。再灌注后3 h心电图显示损伤加重。心、肾、脑、肺组织均显示明显再灌注损伤样病理学改变。结论:成功建立了重复性较好的大鼠全身性缺血-再灌注损伤模型。
[关键词] 缺血-再灌注损伤； 休克； 大鼠； 疾病模型，动物
[中图分类号] R-332; R649.9 [文献标识码] A [文章编号] 1671-7256(2011)02-0172-03
doi：10.3969/j.issn.1671-7256.2011.02.015
Establishment of a rat model of general ischemia-reperfusion injury
FAN Feng-yan, WANG Hong-bo, ZHOU Jing, WEI Shao-ping
(Department of Blood Transfusion, General Hospital of Air-force, Beijing 100142, China)
[Abstract] Objective: To establish a rat model of general ischemia-reperfusion injury. Methords: Thirty Wistar rats achieved ischemia by hemorrhage and achieved reperfusion iniury by infusion of the bled blood. 1 hour after hemorrhage the total bled blood was reinfused into the animals and the rats were killed 3 houes later. Mean arterial pressure(MAP), heart rates, anus temperature and electrocardiogram(ECG) were monitored all the time. Blood samples were taken before hemorrhage and after 3 hours of inspection were analyzed for serum aspartate aminotransferase(AST), alanin aminotransferase(ALT), lactate dehydrogenase(LDH). Heart, kidney, brain and lung samples were taken for histopathological analysis. Results: The results showed that data of MAP, heart rates, anus temperature were normal distribution. Compared with those before hemorrhage the AST, ALT, LDH increased significantly after 3 hours of reperfusion iniury. The changes of ECG after ischemia and reperfusion accorded with ischemia and reperfusion injury. Histopathological changes of heart, kidney, brain and lung after 3 hours of reperfusion accorded with reperfusion injury. Conclusion: A rat model of general ischemia-reperfusion injury was successfully established.
[Key words] ischemia-reperfusion injury； shock； rats； disease models, animal
缺血-再灌注损伤是临床常见的失血性休克后血容量复苏的并发症。单个器官缺血-再灌注损伤模型已经比较成熟,国内外有大量相关文献报道[1-2],但单个器官缺血-再灌注损伤模型往往不能反映临床失血性休克后血容量复苏造成的全身多系统器官损伤,尤其是不适合于评价全身给药对多系统器官缺血-再灌注损伤的防治作用。本实验中我们选用费用低廉、易于饲养的Wistar大鼠,建立了全身性缺血-再灌注损伤的模型,现报道如下。
1 材料与方法 ......
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