高钾致死大鼠心脏热缺血后心脏结构和功能的变化(1)
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摘要：目的研究高钾致死后大鼠不同热缺血后心脏结构和功能的变化，以判定供心的热缺血时间限制。方法32只Wistar大鼠随机分为对照组(A组，热缺血2 min)、热缺血5 min组(B组，热缺血5 min)、热缺血10 min组(C组，热缺血10 min)、热缺血15 min组(D组，热缺血15 min)，每组8只。热缺血结束后建立Langendorff模型：观察心脏复苏情况，测定平均冠脉流量，描记左室内压力波形，计算相应血流动力学指标，进行组织学检查。结果A组、B组心脏复苏显著优于C组、D组，前两组在复灌初始即全部恢复窦性心律，C组有5只心脏在灌注10 min之内自动恢复窦性节律，偶有室早，另外3只初始为室颤，经心脏按压后2只复律，1只持续室颤至试验结束仍未复律。D组复灌初始全部为室颤，经灌注15 min~20 min后，5只心脏恢复窦性节律，另外3只经反复按摩至试验结束仍未复律。A、B、C、D 组血流动力学各项基础值差异有统计学意义，B组与A组比较无统计学意义(P>0.05)，C组、D组结果明显低于A组和B组(P<0.01)。C组与D组各项指标差异无统计学意义(P>0.05)。结论高钾致死大鼠心脏常温热缺血10 min以上不宜用于心脏移植。
关键词:热缺血 心脏移植 高钾 供心
中图分类号：R654T256.2文献标识码：A文章编号：1672-1349(2011)05-0569-02
Heart Function and Myocardial Structure Following Heart Warm-ischemia Caused by Hyperpotassemia in Rats
Zhao Weimao，Liang Zhixing // The First Hospital，Shanxi Medical University(Taiyuan 030001)
Abstract:ObjectiveTo research the heart function and myocardial structure of non-beating heart warm ischemia caused by hyperpotassemia in rats in order to conform the warm-ischemia time limit of the donor heart.MethodsThirty-two Wistar rats were divided into 4 groups randomly:Group A (n=8，warm ischemia 2 min)，group B(n=8，warm ischemia 5 min)，group C (n=8，warm ischemia 10 min)，group D(n=8，warm ischemia 15 min).After the warm ischemia，Langendorff Model was established.The resuscitation of the hearts was observed，and coronary flow was measured.The intra-left ventricular pressure was record and hemodynamic values were calculated.The samples of heart were observed by light microscope.ResultsThe resuscitation in group A and group B was better than that in group C and group D.The hearts in group A and B got sinus rhythms as soon as reperfusion initiated.While ventricular fibrillation (VF) was developed in all hearts in group D.Five out of eight hearts in group D had sinus rhythm 15 min to 20 min after reperfusion started，there were three VF rhythms with continuous compression in this group ......
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