高原环境下REM睡眠剥夺大鼠皮质GRP78的表达及药物干预(1)
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摘要：目的探讨高原环境下大鼠不同时间快速动眼睡眠(REM)期睡眠剥夺(SD)后皮层内质网应激(ERS)标志性分子葡萄糖调控蛋白78(GRP78)的动态变化、神经元凋亡影响及人参皂甙Rd(GS Rd)可能的神经保护作用。方法采用小平台水环境法制作大鼠SD模型，应用免疫组织化学染色检测相关时间点GRP78的动态变化；TUNEL试剂盒检测凋亡细胞。结果在平原(兰州)组，SD后1 d皮层区GRP78蛋白表达开始增高，3 d时达高峰，5 d时与正常睡眠组相比无明显差别。GS Rd，组SD后1 d、3 d、5 d GRP78的表达较生理盐水对照组增高。在对应时间点高原(可可西里)组GRP78的表达均高于平原组。结论高原SD组大鼠GRP78的表达较平原组增高；GS Rd能够提高SD组大鼠GRP78表达；GS Rd可能会通过升高GRP78的表达保护内质网功能，以减轻脑损伤。
关键词：高原 睡眠剥夺 葡萄糖调控蛋白78 皮层 人参皂甙Rd
中图分类号：R741文献标识码：A文章编号：1672-1349(2011)05-0591-02
Expression of GRP78 in Rats with Sleep Deprivation during High-altitude Environment and Intervention
Ma Yajie，Yang Jinsheng，Yang Xiao，et al
The Second Clinical Medical College，Lanzhou University(Lanzhou 730050)
Abstract:ObjectiveTo investigate the dynamic expression of endoplasmic reticulum stress(ERS)GRP78，neuron apoptosis in the rat cerebral contex at different stages of REM sleep deprivation in high altitude environment，and the possible neural protective effect of ginsenoside Rd.MethodsThe sleep deprivation of Wistar rats was induced by employing“flower pot”technique.Expression of GRP78 protein was check with immunohistochemistry staining.Apoptosis cell was studied with TUNEL.ResultsImmunohistochemistry staining results showed that the expressions of GRP78 protein was increased after one day of REM sleep deprivation compared with that in rats with normal sleep in plain group(Lanzhou group) and reached the highest on the normal sleep group.After intervention of ginsenoside Rd，the expression of GRP78 was higher than physiological saline groups after first or third day of REM sleep deprivation.The expression of GRP78 protein was not different from physiological saline groups on the fifth day.At the same time the expression of GRP78 protein in plateau group(Kekexili group) was higher compared with plain group.ConclusionThe expression of GRP78 protein was higher in plateau compared with plain groups.Ginsenoside Rd could increase expression of GRP78 protein.Ginsenoside Rd might lessen brain injuries by increasing expression of GRP78.
Key words:plateau；sleep deprivation；GRP78；cerebral contex；ginsenoside Rd ......
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