清热化瘀方对脑缺血再灌注损伤大鼠神经干细胞增殖的影响(1)
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摘要：目的 观察清热化瘀方对大鼠脑缺血再灌注损伤后神经干细胞增殖的影响。方法 90只大鼠随机分为假手术组、模型组、清热化瘀组3组，采用线栓法制作局灶性脑缺血大鼠(MCAO)模型，观察大鼠缺血损伤后缺血半暗带区的细胞增殖。采用免疫组化法观察脑缺血再灌注后2 d、4 d、7 d、14 d、21 d五个时间点巢蛋白(Nestin)及5-溴脱氧尿苷(BrdU)免疫活性的变化。结果 清热化瘀组大鼠在第2天、第4天、第7天的神经功能缺损评分明显优于模型组(P<0.05)；模型组缺血半暗带Nestin和BrdU阳性表达分别于再灌注7 d和14 d达高峰(P<0.05)，以后表达逐渐减弱；清热化瘀组可以明显增加缺血再灌注后第4天、第7天、第14天大鼠的Nestin阳性细胞表达(P<0.05)；并可以明显增加缺血再灌注后第7天、第14天、第21天大鼠缺血半暗带的BrdU阳性细胞表达(P<0.05)。结论 脑缺血损伤可激活神经干细胞，促使其增殖和表达。清热化瘀方可以促进 MCAO大鼠缺血半暗带神经干细胞的增殖。
关键词：清热化瘀方；脑缺血；神经干细胞；增殖
中图分类号：R743 R289.5 文献标识码：A 文章编号：1672-1349(2011)08-0977-03
Influence of Qingre Huayu Prescription on Neural Stem Cell Proliferation in Rats with Cerebral Ischemia/reperfusion
Hu Yueqiang，Tang Nong，Liu Tai，et al// Department of Neurology，The First Affiliated Hospital，Guangxi University of Traditional Chinese Medicine (Nanning 530023)
Abstract:Objective To observe the effect of Qingre Huayu prescription (QHP) on proliferation of neural stem cells in rats with cerebral ischemia/reperfusion (I/R).Methods Ninety rats were randomly divided into three groups:Sham operation group，middle cerebral artery occlusion (MCAO) group and QHP group.The I/R rat models were established by MCAO to observe the expressionof nestin and 5 Bromo 2 Deoxy Uridine(BrdU) protein by immunochemistry method at 2 d，4 d，7 d，14 d，21 d after I/R.Results Compared with the model group，the neuro-function deficit score in QHP group were obviously better at 2 d ......
摘要：目的 观察清热化瘀方对大鼠脑缺血再灌注损伤后神经干细胞增殖的影响。方法 90只大鼠随机分为假手术组、模型组、清热化瘀组3组，采用线栓法制作局灶性脑缺血大鼠(MCAO)模型，观察大鼠缺血损伤后缺血半暗带区的细胞增殖。采用免疫组化法观察脑缺血再灌注后2 d、4 d、7 d、14 d、21 d五个时间点巢蛋白(Nestin)及5-溴脱氧尿苷(BrdU)免疫活性的变化。结果 清热化瘀组大鼠在第2天、第4天、第7天的神经功能缺损评分明显优于模型组(P<0.05)；模型组缺血半暗带Nestin和BrdU阳性表达分别于再灌注7 d和14 d达高峰(P<0.05)，以后表达逐渐减弱；清热化瘀组可以明显增加缺血再灌注后第4天、第7天、第14天大鼠的Nestin阳性细胞表达(P<0.05)；并可以明显增加缺血再灌注后第7天、第14天、第21天大鼠缺血半暗带的BrdU阳性细胞表达(P<0.05)。结论 脑缺血损伤可激活神经干细胞，促使其增殖和表达。清热化瘀方可以促进 MCAO大鼠缺血半暗带神经干细胞的增殖。
关键词：清热化瘀方；脑缺血；神经干细胞；增殖
中图分类号：R743 R289.5 文献标识码：A 文章编号：1672-1349(2011)08-0977-03
Influence of Qingre Huayu Prescription on Neural Stem Cell Proliferation in Rats with Cerebral Ischemia/reperfusion
Hu Yueqiang，Tang Nong，Liu Tai，et al// Department of Neurology，The First Affiliated Hospital，Guangxi University of Traditional Chinese Medicine (Nanning 530023)
Abstract:Objective To observe the effect of Qingre Huayu prescription (QHP) on proliferation of neural stem cells in rats with cerebral ischemia/reperfusion (I/R).Methods Ninety rats were randomly divided into three groups:Sham operation group，middle cerebral artery occlusion (MCAO) group and QHP group.The I/R rat models were established by MCAO to observe the expressionof nestin and 5 Bromo 2 Deoxy Uridine(BrdU) protein by immunochemistry method at 2 d，4 d，7 d，14 d，21 d after I/R.Results Compared with the model group，the neuro-function deficit score in QHP group were obviously better at 2 d ......
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