超声下观察芎芍胶囊对心肌梗死大鼠缺血心肌血管新生的作用(1)
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摘要：目的 探讨中成药芎芍胶囊对心肌梗死大鼠缺血心肌血管新生作用。方法 采用开胸冠状动脉左前降支的方法建立心肌梗死模型，清洁级(或SPF级)SD大鼠32只(雄性)分为空白组、模型组、芎芍组、丹参组，每组8只，分别用生理盐水、芎芍胶囊、丹参粉末进行灌胃给药，连续给药。6周后采集数据分离样本，通过二维超声观察大鼠心功能指标，以及大鼠心肌组织中外源性血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)的表达。结果 芎芍组、丹参组与模型组比较，左心室收缩末期内径(LVESD)、舒张末期内径(LVEED)、左心室射血分数(LVEF)差异均有统计学意义(P<0.01)，芎芍组、丹参组与AMI组比较，VEGF、bFGF差异均有统计学意义(P<0.01)。VEGF、bFGF与LVEF呈正相关(r值分别为0.813、0.589，P<0.01)。结论 芎芍胶囊较丹参更具有促进心肌梗死大鼠缺血心肌血管新生，提高心肌梗死大鼠心功能，保护心肌缺血性损伤的作用。
关键词：芎芍胶囊；心肌梗死；血管新生
中图分类号：R542.2 R285.5 文献标识码：A 文章编号：16721349(2012)02019102
The Effective Influence of Xiongshao Capsule on Ischemic Myocardium in Rats with Ultrasonography
Chen Yuning,Yan Ping,Lin Jiumao，et alThe Second Affiliated People’s Hospital，Fujian University of Traditional Chinese Medicine(Fuzhou 350003)
Abstract：Objective To explore the Xiongshao capsule(XSC) on angiogenesis in rats with acute myocardial infarction(AMI).Methods Models with myocardial infarction were established by ligation of coronary artery.Thirtytwo rats were divided into four groups:Control group,model group,XSC group,and Danshen group treated with saline water,Xiongshao capsule,Danshen powder for 6 weeks,respectively.Index of the heart function was observed by twodimension ultrasonic sound.Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were studied.Results There were statistical differences in left ventricular endsystolic diameter (LVESD),left ventricular enddiastolic diameter (LVEED),left ventricular ejection fraction (LVEF) between XSC group and Danshen group compared with model group (P<0.01),and VEGF and bFGF were statistical difference compared with AMI group.There was difference between LVEF and bFGF.Conclusion XSC could protect myocardial infarction by improving the heart function and promote the expression of VEGF and bFGF.
Key words:Xiongshao capsule;myocardial infarction;angiogenesis
梗死后心功能的变化与多因素有关，其中很重要的是与新生毛细血管的数量关系密切[1]。以往的研究提示，新生毛细血管的数量与血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)的表达呈正相关。本课题以能显著促进血管新生的芎芍胶囊、丹参颗粒为干预措施，采用结扎大鼠冠状动脉造成急性心肌梗死为缺血心肌实验模型，以超声检查的指标代表心功能，研究心功能与促进毛细血管增生因子间的关系，以比较芎芍胶囊与丹参颗粒促进新生毛细血管的功效。
1 材料与方法
1.1 实验材料
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