【摘要】目的了解乙型肝炎病毒(HBV)基因型流行情况及其临床意义。方法用套式PCR扩增检测的各型乙型肝炎患者和慢性无症状HBV携带者(ASC)共390份血清HBVDNA，进行基因分型。结果大连HBV各种基因型分布比例：基因型C占80.3%，基因型B占7.2%，未分型基因型12.3%，BC混合型占0.3%。HBV基因B较常见于轻型肝病(如慢性肝炎轻度组)，尚无发展至肝硬化者。而基因型C则常见于较重的肝病(如慢性肝炎中重度组)，发展至肝硬化者占相当比例。结论①主要流行的HBV主要为基因型C。②基因型C与较严重的肝脏疾病的发生有关。③发现大连的慢性肝炎存在少量的HBV基因型B+C的混合型。

    【关键词】肝炎 乙型肝炎病毒 基因型

    Distribution of hepatitis B virus genotypes and its clinical significance

    【Abstract】 Objective To understand the distribution of hepatitis B virus genotype and its clinical significance.Methods Nested polymerase chain reaction was used for amplification of HBV DNA in sera of patients with different liver diseases and asymptom carrier.Specimens from 390 subjects were positive for HBVDNA and HBV genotype was determined.Results The prevalence of genotype C was 80.3%, genotype B was 7.2%, unspecified genotype was 12.3%, BC mixed genotype was 0.3%.The prevalence of genotype B showed an increasing trend in patients with tender damaged chronic hepatitis group, no liver cirrhosis patient was found；in contrast ......