摘要：目的研究不同发酵条件对绿色木霉产复合木聚糖酶性能的影响。方法采用固体发酵培养方式，通过改变发酵条件，包括碳源、氮源的种类、浓度及接种量，测定相应条件下发酵产物的酶活性来确定发酵工艺。结果绿色木霉产酶的最佳碳源为玉米芯和麸皮(4∶6)；氮源为硫酸铵(4%)；接种量为1×107个孢子/g培养基。30 ℃固体培养70 h，发酵后用无菌水(pH 5.0)28 ℃浸提3 h测定酶活性。结论在优化的培养条件下，木聚糖酶的最高酶活性达到 267 U/g。

    关键词：绿色木霉；复合木聚糖酶；固体发酵

    中图分类号：Q556+.2文献标识码：A文章编号：1672-979X(2007)03-0005-03

    Condition Optimization on Complex Xylanase by Trichoderma viride in Solid-state Fermentation

    SU Yi-shan, ZHANG Xiao-yuan, ZHU Xi-qiang*, GUO Xue-ping, LING Pei-xue

    (Institute of Biopharmaceuticals of Shandong Province, Jinan 250108, China)

    Abstract：Objective To study the effects of fermentation conditions on the production of complex xylanase by Trichoderma viride. Methods An optimized incubation condition in solid-state fermentation was achieved by determining the enzyme activity when altering culture conditions, such as types and concentrations of carbon source, nitrogen source and inoculum volume. Results The optimized condition in solid-state fermentation ofTrichoderma viride was as follows：corncob and wheat bran(mass ratio 4:6) as carbon sources ......