黄芪固态发酵中有效成分的变化
摘要:目的研究高温灭菌对黄芪有效成分含量的影响,考察灵芝在含黄芪基质的生长情况。方法紫外分光光度法测定黄芪多糖、总黄酮和总皂苷的含量。结果高温处理后测得多糖和黄酮含量分别增大了56.3%和43.8%,黄芪皂苷含量降低了63.2%。黄芪固态发酵前10 d 菌质多糖含量变化剧烈,30 d 后多糖含量基本不变,从而确定为发酵终点。结论高温灭菌对黄芪有效成分含量有一定影响,灵芝在含黄芪基质长势良好。
关键词:高温灭菌;黄芪;固态发酵;多糖
中图分类号:R927.7文献表识码:A文章编号:1672-979X(2007)03-0008-03
Changes of Effective Components of Radix Astragali by Solid Fermentation
YU Shuai-Lu , HE Xun , LU Li-Xia , XIONG Xiao-Hui
(Nanjing University of Technology, Nanjing 210009, China)
Abstract:Objective To study the effect of sterilizing at high temperature on the content of effective components in Radix Astragali and the growth of Ganoderma lucidum in medium containing Radix Astragali. Methods The contents of polysaccharides, total flavonoids and saponins were determined by UV-spectrophotometry. ResultsThe contents of polysaccharides and flavonoids increased by 56.3% and 43.8% respectively, and the content of saponins decreased by 63.2% after high temperature processing. During the first ten days, the content of fungus polysaccharides changed violently. But the content change was very small after thirty days, thus, the end point of fermentation was determined. Conclusion Sterilizing at high temperature has some impact on the content of effective components in Radix Astragali; Ganoderma lucidum grows well in medium containing Radix Astragali. ......
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