摘要：目的探讨从蜂王浆中提取蜂王酸(10-HAD)的工艺。方法二次有机溶剂提取，二次调pH，用高效液相色谱法对10-HDA进行定性分析。结果蜂王浆中10-HDA的最佳提取条件为：蜂王浆水溶液pH 10，加入乙醚提取杂质，再调pH 2加入乙醚提取10-HDA。结论此法可从蜂王浆中高效提取10-HDA。

    关键词：蜂王浆；蜂王酸；提取；高效液相色谱法；测定

    中图分类号：S896.3 文献标识码：A 文章编号：1672-979X(2008)03-0030-03

    Extraction Study on 10-HDA from Royal jelly

    WANG Wen-feng1, XU Ling1, WANG Teng-fei1, MEI Ying-jie2, WANG Rui-ming1*

    (1.Shandong Institute of Light Industry, Jinan 250353, China; 2. Zibo Institute of Product Quality Supervision & Inspection , Zibo 255000, China)

    Abstract：Objective To discuss the technique of extraction of 10-HDA from royal jelly. Methods The HPLC was used for the qualitative analysis of 10-HDA with two organic solvents used and pH adjusted twice. ResultsThe best extraction condition of 10-HDA from the royal jelly was as follows: first adjusting the pH of the solution to 10, then adding ether for the extraction of impurity; secondly adjusting the pH to 2, then adding ether for the extraction of 10-HDA . Conclusion This method can be used for the high effective extraction of 10-HAD from royal jelly. ......