摘要：目的从泡菜汁中分离产凝集素乳杆菌并鉴定。方法通过凝集实验筛选产凝集素的乳杆菌，用聚合酶链式反应(PCR)扩增16S rDNA可变区基因鉴定产凝集素乳杆菌。结果此菌株呈革兰阳性，短杆状，产乳酸。通过同源性比较，此菌株与Lactobacillus plantarum strain p215的同源性为99 %。结论分离得到了产凝集素的菌株，鉴定表明此菌株为植物乳杆菌。

    关键词：泡菜；乳杆菌；分离；鉴定；凝集活性；DNA；核糖体；序列分析

    中图分类号：Q513+.2文献标识码：A文章编号：1672-979X(2008)09-0009-03

    Screening of Lectin-producing Lactobacillus and Identification of its 16S rDNA Gene

    XU Jin1, ZHAO Sheng-lan2, CHEN Chao-yin1*

    (1. Institute of Bioresource R&D of Kunming University of Science and Technology-Tsinghua University, Kunming 650224, China; 2. Department of Applied Pharmacy, Yunnan Institute of Traditional Chinese Medicine, Kunming 650220, China) ......