NADPH氧化酶4在高糖促进HUVECs内活性氧产生机制中的作用(1)
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[摘要] 目的：研究在高浓度葡萄糖刺激状态下，人脐静脉内皮细胞(HUVECs)内活性氧的主要来源。方法：Ⅱ型胶原酶消化法分离培养人脐静脉内皮细胞，流式细胞术检测细胞内活性氧的生成，RT-PCR及Western blot检测细胞内NADPH氧化酶4(NOX4)的表达。结果：高糖处理HUVECs后细胞内ROS升高、NOX4表达明显上调；NOX抑制剂DPI能够抑制NOX4表达的上调和细胞内ROS的升高。结论：高糖处理HUVECs时细胞内ROS升高主要来源于NOX4。
[关键词] 葡萄糖；HUVECs；活性氧；NADPH氧化酶4
[中图分类号] R34[文献标识码]A [文章编号]1673-7210(2008)06(b)-011-03
The role of NADPH oxidase in intracellular ROS generation stimulated by high concentration of glucose
JIN Juan
(Department of Medicine, Changde Vocational Technical College, Changde 415000, China )
[Abstract] Objective: To study the main source of ROS generated in human umbilical vein endothelial cells (HUVECs) while stimulated by high concentration of glucose.Methods:Human umbilical vein endothelial cells was isolated by type Ⅱ collagenase, Intracellular ROS generation was measured by flow cytometry, NOX4 expression in HUVECs was detected by RT-PCR and Western blot ......
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