反相高效液相蒸发光散射检测法测定三金软胶囊中羟基积雪草苷含量高永艳 黎永洁
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[摘要] 目的：建立测定三金软胶囊中羟基积雪草苷含量的方法。方法：采用反相高效液相色谱法测定羟基积雪草苷含量。色谱条件为Diamonsil C18柱(150 mm×4.6 mm，5 μm)，流动相为甲醇-水(50∶50)，检测器为蒸发光散射检测器。结果：羟基积雪草苷在1.006~10.064 μg的范围内，进样量与峰面积呈良好的线性关系(r=0.999 2)，精密度、8 h内稳定性、重复性均符合要求，平均回收率为100.16%(RSD=2.28%)。结论：该含量测定方法灵敏、准确，重复性好，专属性强，适合羟基积雪草苷的含量测定。
[关键词] 三金软胶囊；高效液相色谱法；蒸发光散射检测器；羟基积雪草苷
[中图分类号] R927.2[文献标识码]A [文章编号]1673-7210(2008)10(b)-026-02
Determination ofmadecassoside in Sanjin Soft Capsulae by RP-HPLC-ELSD
GAO Yong-yan，LI Yong-jie
(Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou510120,China)
[Abstract] Objective：To determine the content of madecassoside in Sanjin Soft Capsulae. Methods：HPLC method was used to determine the content of madecassoside. The column was Diamonsil C18(150 mm×4.6 mm,5 μm),the mobile phase was methanol-water(50∶50),and the evaporative light-scattering detector(ESLD) was used as detector ......
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