纯中药制剂“卡宁”对肝癌细胞的作用(1)
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[摘要] 目的：观察抗癌中药“卡宁”对体外培养的肝癌细胞株BEL-7402细胞的凋亡抑制基因bcl-2的影响，探讨其抗肿瘤治疗的作用机制。方法：取体外培养的对数生长期BEL-7402细胞，随机分为4组：对照组，5-氟尿嘧啶组，含药“卡宁”血清1组(10%含药血清组)和含药“卡宁”血清2组(15%含药血清组)；采用MTT比色法观察不同浓度含药血清对体外培养的人肝癌细胞株BEL-7402细胞的抑制能力，PCR检测其对凋亡抑制基因bcl-2的影响。结果：PCR结果显示，“卡宁”1、2组bcl-2的活性明显低于对照组(P=0.038)；MTT结果显示，细胞生长抑制率随含药血清作用时间及浓度的增加而增大(P<0.05)，15%含药血清72 h的作用最强。结论：对肿瘤细胞bcl-2的抑制可能是抗癌中药“卡宁”诱导肿瘤细胞凋亡作用机制的一部分。
[关键词] bcl-2；中草药；肝脏肿瘤
[中图分类号] R735.7[文献标识码]A [文章编号]1673-7210(2009)01(a)-014-03
Effect of chinese medicine "Caning" on hepatic cancer cells
MAO Xiao-ling, PAN Su-ying, ZHANG Da-peng
(The First Affiliated Hospital of Guangzhou Medical College, Guangzhou510120, China)
[Abstract] Objective: To observe the effects of anticancer chinese medicine "Caning" on the anti-apoptosis gene bcl-2 of in vitro cultured human hepatic cancer cell line BEL-7402 cells, and investigates its anticancer therapeutic mechanism. Methods: In vitro cultured BEL-7402 cells in a logarithmic growth phase were randomly divided into 4 groups: control group, 5-Fluorouracil treated group, serumgroup 1 containing "Caning" (10% pastille serum) and serumgroup 2 containing "Caning"(15% pastille serum); then MTT assay was employed to detect the inhibitory ability of serums containing different concentrations of "Caning" to in vitro cultured human hepatic cancer cell line BEL-7402 cells, and PCR was used to detect the effect of "Caning" on the anti-apoptosis gene bcl-2. Results: Results of in vitro experiment suggested that, the bcl-2 gene activity in serum group 1 and 2 containing "Caning" was significantly lower than that of control group (P=0.038); result of MTT assay indicated that the cell growth inhibitory rate was increased along with the increase of the action time and concentration of serum containing "Caning"(P<0.05), and the 15% pastille serum showed the strongest inhibitory effect at 72 h. Conclusion: "Caning" could significantly inhibit the growth of hepatic cancer cells partly by inhibiting the anti-apoptosis gene bcl-2 ......
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