白藜芦醇对大鼠脑缺血再灌注损伤抗炎机制研究(1)
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[摘要] 目的：研究白藜芦醇对大鼠局灶性脑缺血再灌注损伤抗炎作用机制。方法：大鼠脑缺血再灌注模型完成后，缺血2 h再灌注24 h，其中白藜芦醇大剂量组(40 mg/kg)、小剂量组(10 mg/kg)术前7 d分别腹腔注射2 ml/kg白藜芦醇无菌溶液，模型组腹腔注射2 ml/kg生理盐水，1次/d，各组大鼠均于手术前15 min加注1次。观察不同剂量的白藜芦醇对脑组织一氧化氮(NO)含量、髓过氧化物酶(MPO)活性和脑组织神经细胞的细胞间黏附分子-1(ICAM-1)蛋白的表达的影响。结果：白藜芦醇可显著减轻大鼠脑缺血再灌注损伤的行为评分，显著降低脑组织中NO含量和MPO的活性，差异有统计学意义(P<0.05)；ICAM-1蛋白的阳性表达率降低，差异有统计学意义(P<0.05)。结论：白藜芦醇对大鼠脑缺血再灌注损伤有保护作用，作用机制之一可能与其抑制炎性反应有关。
[关键词] 白藜芦醇；脑缺血再灌注损伤；细胞间黏附分子-1；髓过氧化物酶；一氧化氮
[中图分类号] R965 [文献标识码]A [文章编号]1673-7210(2009)01(a)-041-03
Study on anti-inflammatory mechanisms of resveratrol on cerebral ischemia reperfusion injury of rats
WANG Feng1, CAI Hong-bin2, ZHANG Yi-jun3*
(1. Laboratory of Pharmacy, Weifang Medical University, Weifang261053, China; 2. Zhoucun Psychiatric Hospital in Zibo City, Shangdong Province, Zibo255300，China； 3. Functional Laboratory, Weifang Medical University,Weifang261053, China)
[Abstract] Objective: To investigate the anti-inflammatory mechanisms of resveratrol on cerebral ischemia reperfusion injury (CIRI) of rats. Methods: The model of MCAO was established by intraluminal suture technique. After 2 h of occlusion and 24 h of reperfusion, the rats of the high dose resveratral (40 mg/kg) group and low dose resveratrol (10 mg/kg) group were injected by intraperitoneal injection for 7 days, respectively, and the control group were given normal saline(2 ml/kg)，one time a day, and all groups were given a more time in 15 minutes before operation. The contents of NO and activities of MPO in brain tissue of ischemic hemisphere were measured; the expression of ICAM-1 in the cortex were measured by immunohistochemical method. Results: The neurological scores of rats treated with resveratrol was reduced apparently compared with model group rats (P<0.05); there were apparent reduction of the contents of NO and marked decrease of MPO inResveratrol group compared with the I/R model group (P<0.05); compared with I/R model group, the expression of ICAM-1 was down-regulated obviously in resveratral group (P<0 ......
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