CD4+CD25+调节性T细胞及其相关基因Foxp3在非小细胞肺癌患者外周血中的变化(1)
 |
| 第1页 |
参见附件(1519KB，3页)。
[摘要] 目的：探讨非小细胞肺癌患者外周血中CD4+CD25+调节性T细胞(regulatory T cell, Treg)数量变化及其相关基因Foxp3 mRNA的表达改变，探讨CD4+CD25+调节性T细胞在诱导肿瘤免疫耐受中的作用。方法：采用流式细胞术检测患者外周血中CD4+CD25+调节性T细胞数量变化；采用半定量RT-PCR方法检测肺癌患者外周血PBMC中Foxp3 mRNA的表达水平。选取30名非肿瘤患者作为对照。结果：肺癌患者外周血中CD4+CD25+调节性T细胞数量明显高于正常组(P<0.05)；Foxp3 mRNA在肺鳞癌和肺腺癌患者外周血PBMC中的表达明显增强(P<0.05)。结论：CD4+CD25+调节性T细胞可能在肺癌免疫耐受中发挥重要作用。
[关键词] CD4+CD25+；调节性T细胞；Foxp3；肺癌
[中图分类号] R734.2[文献标识码]A [文章编号]1673-7210(2009)03(a)-023-03
The changes of CD4+CD25+ T cells and expression of Foxp3 mRNA in peripheral blood mononuclear cells from patients with non-small-cell lung carcinoma
XU Lang, WANG Yu-bin, LIU Dan, LI Yu-hong, YAN Dan, SUN Yuan-chang, LIU Li-min
(Wuhan University of Science and Technology, Wuhan430065, China)
[Abstract] Objective: To investigate the changes of CD4+CD25+ T cells and expression of Foxp3 mRNA in peripheral blood mononuclear cells from patients with non-small-cell lung carcinoma. Methods: Flow cytometry was used to analyze the contents of CD4+CD25+ regulatory T cells in peripheral blood from the patients with non-small-cell lung carcinoma and patients without cancer as control. The expression of Foxp3 mRNA in peripheral blood mononuclear cells was detected by RT-PCR. Results: The population of CD4+CD25+ regulatory T cells in peripheral blood from the patients with non-small-cell lung carcinoma was significantly higher in comparison with that of control (P<0.05). The expression of Foxp3 mRNA in patients with lung carcinoma was also significantly higher in comparison with that of control (P<0.05). Conclusion: CD4+CD25+ regulatory T cells and its related Foxp3 may play an important role in the immune of tumor.
[Key words] CD4+CD25+; Regulatory T cells; Foxp3; Lung carcinoma
肺癌是当今世界对人类健康危害最大的肿瘤之一，且其发病率有不断上升的趋势。预计到2025年，每年将有90万人死于肺癌，中国将成为肺癌第一大国。因此，对肺癌的研究意义重大。
CD4+CD25+调节性T细胞(regulatory T cell, Treg)是健康个体T细胞库的组成部分，占CD4+细胞的5%~10%。该细胞群具有抑制免疫效应的作用，能特异性抑制T细胞对外源和自体抗原的免疫反应，因此在维持对自身成分免疫耐受的同时，也可能阻止机体对自体同源肿瘤细胞的免疫。目前普遍认为，CD4+CD25+Treg很可能影响免疫系统对肿瘤抗原的应答，从而影响肿瘤的发生和发展。Foxp3是特异性地表达于CD4+CD25+Treg表面的一种转录因子，可作为该类细胞的特异标志，并调节其发育和功能。
本文通过检测非小细胞肺癌患者外周血中CD4+CD25+Treg的数量变化及其相关基因Foxp3 mRNA的表达，探讨CD4+CD25+Treg与非小细胞肺癌发生、发展的关系。
1 对象与方法
1.1 研究对象 ......
您现在查看是摘要介绍页，详见PDF附件(1519KB，3页)。