葛根素与石杉碱甲改善缺血-再灌注小鼠学习记忆(1)
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[摘要] 目的：观察葛根素(Pur)与石杉碱甲(Hup-A)单用及合用对缺血-再灌注(I/R)小鼠学习记忆的影响，并探讨其作用机制。方法：反复夹闭小鼠颈总动脉制备I/R模型，将小鼠随机分为5组：假手术组(Sham)、缺血-再灌注模型组(I/R)、石杉碱甲组(Hup-A)、葛根素组(Pur)及葛根素加石杉碱甲联合用药组(Pur+Hup-A)。分别以跳台法评价学习记忆能力，以高效液相法(HPLC)测定单胺类神经递质及其代谢产物的含量，以流式细胞仪技术检测细胞凋亡。结果：Pur(120 mg/kg)或Hup-A(400 μg/kg)都可提高I/R小鼠的学习记忆成绩，Pur组的作用优于Hup-A组，联合用药组(Hup-A 200 μg/kg+Pur 60 mg/kg)作用最强；各用药组都可降低大脑皮层细胞凋亡率，升高大脑皮层某些单胺类递质水平，其中，联合用药组的大脑皮层细胞凋亡率低于Hup-A组，大脑皮层高香草酸(HVA)水平高于Pur组。结论：在该给药剂量下，Pur改善I/R小鼠学习记忆能力的作用强于Hup-A，两药的作用机制与减少大脑皮层细胞凋亡、调节大脑皮层单胺类递质水平有关，联合用药组作用最强。
[关键词] 葛根素；石杉碱甲；缺血-再灌注；学习记忆
[中图分类号] R285.5[文献标识码]A [文章编号]1673-7210(2009)03(a)-025-03
Puerarin and Huperzine A improve learning memory dysfunction induced by ischemia-reperfusion in mice
SHI Rui-li, SHEN Shu-ping, PANG Dong-wei, WANG Li-jun, ZHAO Li-juan, GAO Li-jun
(Baotou Medical College, Baotou014010, China)
[Abstract] Objective: To observe the effects of Puerarin (Pur) and Huperzine A (Hup-A) on learning memory dysfunction induced by ischemia-reperfusion (I/R) in mice and explore its mechanism. Methods: I/R model in mice was performed by repetitious ligation of bilateral common carotid artery, and mice were randomly divided into five groups: Sham group, I/R group, Hup-A group, Pur group and Pur+Hup-A group.Learning memory ability was evaluated by step-down test. Monoamine nervous transmitter level and cortex neuron apoptosis were measured by HPLC and flow cytometry respectively. Results: Both Pur and Hup-A improved learning memory ability of I/R mice, the effect of Pur group was better and the effect of combination group was the most significant. Both Pur and Hup-A decreased neuron apoptosis and raised content of monoamine nervous transmitter of I/R mice. The cortex neuron apoptosis rate in combination group was lower than that in Hup-A group，the homovanillic acid (HVA) level of cerebral cortex in combination group was higher than that of Pur group. Conclusion: Learning memory dysfunction induced by I/R in mice could be improved by Pur and Hup-A, the effect of Pur was better than that of Hup-A with administered dose, the mechanisms of drugs might be related to reduction of neuron apoptosis and regulating of the monoamine nervous transmitter level in cerebral cortex, combination of Pur and Hup-A resulted in the most obvious effects ......
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