雌激素对去卵巢大鼠局灶性脑缺血的保护作用及对Bck-2蛋白表达的影响(1)
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[摘要] 目的：研究雌激素的脑保护作用及对Bcl-2蛋白表达的影响，探讨其脑保护作用的机制。方法：将雌性Wistar大鼠去卵巢后分别给予不同剂量的雌激素，连续6 d后制成大脑中动脉闭塞模型，于缺血24 h断头取脑。观察脑梗死灶的面积和Bcl-2蛋白的表达。结果：各给药组与缺血对照组比较，梗死灶面积均减小(P<0.01)，Bcl-2阳性细胞值均明显增加(P<0.01)。在一定范围内，大鼠血雌激素浓度和Bcl-2阳性细胞值呈正相关(r=0.41，P<0.05)。结论：雌激素对去卵巢大鼠缺血性脑损伤具有脑保护作用，这可能是其上调了Bcl-2蛋白表达所致。
[关键词] 17β-雌二醇；去卵巢大鼠；局灶性脑缺血；脑保护作用；Bcl-2
[中图分类号] R743.31[文献标识码]A [文章编号]1673-7210(2009)05(b)-009-03
Protection of estrogen in OVX female rats on focal cerebral ischemia and its correlation with the expression of Bcl-2
LI Runhui1, HE Zhiyi2
(1.Department of Neurology, Affiliated Central Hospital of Shenyang Medical College, Shenyang110024, China; 2.Department of Neurology, the First Affiliated Teaching Hospital of Chinese Medical University, Shenyang110001, China)
[Abstract] Objective: To study the cerebral protective effect of estrogen and its correlation with the expression of bcl-2, and further investigate the mechanism of the cerebral protective effect of estrogen. Methods: The OVX female Wistar rats were given different doses of estrogen each day. After 6 days they were induced by right middle cerebral artery for 24 hours and then killed to study their infarction area and the expression of protein Bcl-2. Results: Compared with the ischemic control group, all the estrogen-treated groups had significantly less infarction area (P<0.01) and higher value of Bcl-2 positive cells(P<0.01). Within a certain time, there was a positive correlation between the plasma density of estrogen and the value of Bcl-2 positive cells of the rats (r=0.41, P<0.05). Conclusion: Estrogen can provide a cerebral protective effect against cerebral ischemic injury in OVX female rats, which is highly associated with the up-regulation of expression of protein Bcl-2.
[Key words] 17β-estrogen; OVX female rat; Focal cerebral ischemia; Cerebral protection effect; Bcl-2
流行病学资料表明，绝经前的女性比同年龄男性脑卒中的发生率低，且脑卒中的严重程度远低于同年龄男性和绝经后女性[1-2]，但绝经后女性脑卒中的发生率迅速增高[3-5]。在对女性绝经后雌激素替代治疗的研究中发现，替代治疗可以使发生脑卒中的相对危险度下降，与脑卒中有关的死亡率亦下降[4-5]。由此，人们推测雌激素具有脑保护作用 ......
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