补阳还五汤对局灶性脑缺血大鼠脑内神经细胞黏附分子表达的影响(1)
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[摘要] 目的：探讨补阳还五汤对局灶性脑缺血大鼠脑内神经细胞黏附分子(NCAM)表达的影响。方法：采用大脑中动脉阻塞法复制脑缺血模型，动物随机分为正常组、假手术组、模型组和补阳还五汤组，各组分别给予不同处理，分别于12 h，1，3，7，14 d处死动物，采用免疫组织化学法观察NCAM的表达。结果：在正常脑内有少量NCAM阳性细胞，缺血后表达增加，于术后第3天达高峰，补阳还五汤组能显著提高NCAM的表达，在第7、14天模型组与补阳还五汤组差异有显著性(P<0.01)。结论：补阳还五汤增强脑缺血后NCAM的表达是其促进神经再生和功能恢复的可能机制之一。
[关键词] 脑缺血/中医药疗法；神经细胞黏附分子；补阳还五汤
[中图分类号] R743.31[文献标识码]A [文章编号]1673-7210(2009)05(b)-033-03
Effects of Buyanghuanwu Decoction on expression of neural cell adhesion molecule in rat models of focal ischemia
ZHU Chuanxiang, LI Wubin, CHEN Xuemei, LIU Baiyan, CAI Guangxian
(TCM Internal Medicine Laboratory, Hunan University of Traditional Chinese Medicine, Changsha 410007, China)
[Abstract] Objective: To investigate the effect of Buyanghuanwu Decoction(BYHW) on expression of neural cell adhesion molecule (NCAM) in rat models of cerebral ischemia. Methods: Brain ischemia was induced by middle cerebral artery occlusion. The rats were randomly divided into four groups, including normal group, sham-operated group, model group and BYHW Decoction group. Rats in each group were given different treatments and killed at 12 h, 1, 3, 7 or 14 d after operation. NCAM expression were measured by immunohistochemical staining. Results: There were a few NCAM positive cells in normal brain, expression of neural cell adhesion molecule was enhanced immediately after ischemia and reached peak at 3 d after ischemia; BYHW Decoction group increased the number of NCAM positive cells. This increase was statistically significant at 7 and 14 d after ischemia and treatment (P<0.01). Conclusion: BYHW Decoction is capable of regulating expression of NCAM in ischemic brain, that may be a neurogenic mechanism of BYHW Decoction.
[Key words] Cerebral ischemic/TCM therapy; Neural cell adhesion molecule; Buyanghuanwu Decoction
神经细胞黏附分子(neural cell adhesion molecule，NCAM)是介导细胞黏附和识别的一种细胞表面糖蛋白，属于细胞黏附分子免疫球蛋白超家族，与神经损伤后组织修复密切相关[1]。补阳还五汤是中医学治疗缺血性中风的传统名方，大量研究表明，补阳还五汤能恢复中风患者的神经功能[2]，具有促进脑缺血后神经再生的作用[3]。那么，补阳还五汤是否通过上调NCAM表达而影响神经再生，本文就此进行探讨。
1 材料与方法
1.1 实验动物
3月龄雄性清洁级Wistar大鼠，280～300 g，湖南中医药大学实验动物中心提供 ......
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