非诺贝特对胰岛素抵抗大鼠血清脂联素及肿瘤坏死因子-α的影响(1)
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[摘要] 目的：观察非诺贝特对胰岛素抵抗(IR)大鼠血清脂联素、肿瘤坏死因子-α(TNF-α)的影响，探讨非诺贝特改善IR的机制。方法：36只Wistar大鼠随机分为正常对照组(12只)和高脂组(24只)，分别给予普通饮食和高脂饮食；6周后高脂组大鼠分为非诺贝特组(F组)和高脂对照组(HF组)，每组12只，分别给予非诺贝特和蒸馏水灌胃4周。以高胰岛素-正常葡萄糖钳夹技术评价胰岛素敏感性；ELISA法检测血清脂联素和TNF-α的水平。结果：HF组大鼠血清脂联素水平明显降低(P<0.05)，而TNF-α水平显著升高(P<0.01)，胰岛素敏感性显著下降(P<0.01)。非诺贝特治疗后，血清脂联素水平明显升高，而TNF-α水平明显下降(P<0.05)，胰岛素敏感性显著增加(P<0.01)。结论：非诺贝特增加IR大鼠血清脂联素水平而降低TNF-α水平，改善IR。
[关键词] 非诺贝特；胰岛素抵抗；脂联素；肿瘤坏死因子-α
[中图分类号] R446.1[文献标识码]A [文章编号]1673-7210(2009)06(c)-024-02
Effects of Fenofibrate on the serum levels of adiponectin and tumor necrosis factor α in rats with insulin resistance
LI Jie, ZHAO Weihua, HU Jian*
(Department of Cardiology, the First Affiliated Hospital of China Medical University, Shenyang 110001, China)
[Abstract] Objective: To observe the effects of Fenofibrate on the serum levels of adiponectin and tumor necrosis factor α (TNF-α) in insulin-resistant rats, and investigate the mechanism of Fenofibrate in improving insulin resistance. Methods: 36 Wistar rats were randomly divided into two groups: normal control group (n=12) and high-fat group (n=24), they were fed with standard diet and high-fat diet respectively. Six weeks later, the high-fat group was randomly divided into another two groups: fenofibrate-treated group (F group, n=12) and high-fat control group (HF group, n=12), they were gastrically administrated with Fenofibrate and distilled water respectively for four weeks. Euglycemic-hyperinsulinemic clamp was performed to estimate the insulin sensitivity. Serum concentration of adiponectin and TNF-α was measured by ELISA assay. Results: Serum adiponectin concentration was decreased (P<0.05) and TNF-α concentration was increased (P<0.01) in HF group, and insulin sensitivity was decreased (P<0.01). After Fenofibrate treatment, the serum adiponectin concentration was increased and TNF-α concentration was decreased (P<0.05) with insulin sensitivity increased (P<0.01). Conclusion: Fenofibrate treatment may increase the serum level of adiponectin and decrease that of TNF-α in rats with insulin resistance. Fenofibrate treatment may improve insulin resistance ......
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