单双波长在生物活性测定中的比较研究
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[摘要] 目的:通过比较研究,确定酶标仪单双波长哪个更适于生物制品活性测定实验。方法:样品通过多次实验单双波长扫描,比较得到的实验结果,进行统计分析。结果:选用双波长可以排除其他因素对结果的影响,实验结果的标准差和离散系数均较低。结论:单双波长法更适于生物活性测定实验,提高结果的准确度。
[关键词] 酶标仪;单波长;双波长
[中图分类号] R927.2[文献标识码] B[文章编号] 1673-7210(2010)04(b)-052-02
The contrast research on single and dual wavelength detection about bioactivity
ZHAO Hong
(Beijing SL Pharmaceutical Co., Ltd., Beijing 100041, China)
[Abstract] Objective: To choose a suitble method of bioactivity detection by making a contrast research, either microplate reader single wavelength or dual wavelength. Methods: Make many experiments of single wavelength and dual wavelength, compared all results, then analyzed statistically. Results: Choose dual wavelength detection, suitable for bioactivity detection, less SD and CV%. Conclusion: Single and dual wavelength are more accuracy and reduce interference factors ......
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