抑癌基因FHIT和PTEN在肝细胞癌中的表达及其意义(1)
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[摘要] 目的:探讨FHIT和PTEN基因在肝细胞癌(HCC)中的表达及其意义。方法:采用免疫组化Envision二步法检测FHIT蛋白及PTEN蛋白在44例HCC组织及10例正常肝组织中的表达。结果:44例肝细胞癌组织中FHIT和PTEN蛋白的阴性表达率分别为38.6%(17/44)和47.7%(21/44),10例正常肝组织中FHIT和PTEN蛋白的阴性表达率均为0,两种抑癌基因在肝细胞癌组织与正常肝组织中的阴性表达率之间差异有统计学意义(P<0.05)。在HCC中FHIT基因的阴性表达率与HCC的病理分级、TNM临床分期和肿瘤直径有关(P<0.05),与AFP水平、HBsAg(+)和有无包膜无关(P>0.05)。PTEN蛋白的阴性表达率与病理分级显著相关(P<0.05),与TNM分期、肿瘤直径、AFP水平、HBsAg(+)和有无包膜无关(P>0.05)。相关性分析发现,FHIT和PTEN在HCC中的阴性表达呈正相关(rs=0.427,P<0.01)。分别比较FHIT和PTEN在HCC中蛋白阳性和阴性表达者的术后1、3年生存率,差异有统计学意义(P<0.05)。结论:FHIT和PTEN蛋白的异常表达与HCC的发生、发展有关,两者可能存在协同作用。FHIT可能是判断HCC恶性程度、侵袭能力和不良预后的重要指标,PTEN可能是判断HCC恶性程度和不良预后的指标。
[关键词] FHIT;PTEN;肝细胞癌;免疫组织化学
[中图分类号] R735.7[文献标识码]A [文章编号]1673-7210(2010)05(a)-008-05
Expression and significance of anti-oncogene FHIT and PTEN in primary hepatocellular carcinoma
QI Ming1, CHEN Guolin2, QUAN Baoku1
(1.Department of the First General Surgery, the First Hospital of Harbin, Harbin 150010, China; 2.Department of Hepatobiliary Surgery, Affiliated Tumor Hospital of Harbin Medical University, Harbin150040, China)
[Abstract] Objective: To investigate the expression and significance of FHIT and PTEN in hepatocellular liver cancer (HCC). Methods: The expressions of FHIT and PTEN in 44 cases of HCC and 10 cases of normal liver tissues were detected by immunohistochemical Envision two-step method. Results: The negative expression rate of FHIT and PTEN were 38.6% (17/44) and 47.7% (21/44) respectively in 44 cases of HCC tissues and 0 in 10 cases of normal liver tissues, expression of each anti-oncogene showed significant difference between HCC tissues and normal liver tissues (P<0.05). The negative expression rate of FHIT in HCC was significantly associated with pathological grade, TNM stage and tumor diameter (P<0.05), but was not associated with AFP level, HBsAg (+) or integral capsule (P>0 ......
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