乙型肝炎患者HBV-DNA定量检测与HBV血清学标志组合的关系(1)
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[摘要] 目的:了解HBV感染的不同血清学组合的HBV-DNA阳性情况。方法:对324例血清同时进行ELISA方法测定HBV-M及荧光定量PCR法检测HBV-DNA。结果:HBsAg(+)组HBV-DNA阳性率为78.4%(254/324),HBsAg(-)组HBV-DNA阳性率为4.8%(5/103),两组间差异有统计学意义(P<0.05)。HBsAg(+)HBeAg(+)HBcAb(+)血清HBV-DNA阳性率为100.0%(120/120),平均含量为1.61×107拷贝/ml;HBsAg(+)HBeAb(+)HBcAb(+)血清HBV-DNA阳性率为47.0%(76/162),平均含量为3.42×104拷贝/ml;HBsAg(+)HBcAb(+)血清HBV-DNA阳性率为69.0%(29/42),平均含量为6.32×103拷贝/ml;HBsAb(+)HBeAb(+)HBcAb(+)血清HBV-DNA阳性率为5.2%(5/96),平均含量为4.12×101拷贝/ml。结论:定量PCR可真实反映HBV感染、复制及病程变化,对乙型肝炎临床诊断及治疗均有较大的指导意义。
[关键词] 乙型肝炎病毒;DNA;ELISA
[中图分类号] R512.6+2[文献标识码] B[文章编号] 1673-7210(2010)06(b)-064-02
The relationship between quantitative detection of HBV-DNA and its serological markers
PENG Jiliang1, WEN Xiuming1, XU Ruihuan2, SHEN Cishi1, HE Chunhui1
(1.Shenzhen Longgang Blood Station, Shenzhen 518172, China; 2. Longgang Central Hospital of Shenzhen City, Shenzhen 518116, China)
[Abstract] Objective: To study the HBV-DNA positive rates under its different serological groups. Methods: 324 HBV serum were detected by ELISA and quatitative PCR technique. Results: The HBV-DNA positive rate was 78.4% (254/324) in HBsAg positive group,and 4.8% (5/103) for HBsAg negative group, the differences between the two groups were statistically significant (P<0.05). 100.0% positive rate of HBV-DNA was to HBsAg(+) HBeAg(+) HBcAb (+) serum about average 1.61×107 copies/ml, 47.0% (76/162) to HBsAg(+)HBeAb(+)HBcAb(+) about 3.42×104 copies/ml, 69.0% (29/42) to HBsAg(+)HBcAb(+) about average 6.32×103 copies/ml, 5.2%(5/96) to HBsAb(+)HBeAb(+)HBcAb(+) about average 4.12×101 copies/ml. Conclusion: Quantitative PCR can really reflect HBV infection and duplication and disease progress, it provides much more information for HBV diagnosis and therapy.
[Key words] Hepatitis B virus; Deoxyribonucleic acid; Ensyme linked immunosorbent assay
近年来随着分子生物学技术的发展及其在临床应用方面的推广,应用实时荧光定量聚合酶链反应检测 HBV 病毒载量已成为目前临床诊断 HBV 感染的常用指标,进一步提高了检测的质量 ......
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