慢性乙肝与重症乙肝患者血清代谢组比较(1)
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[摘要] 目的:应用基于核磁共振氢谱(nuclear magnetic resonance 1H,1H-NMR)的代谢组学方法比较慢性乙肝和重症乙肝患者血清代谢组。方法:对慢性乙肝患者血清(20例)和重症乙肝患者血清(11例)进行核磁共振氢谱检测,应用主成分分析方法找出慢性乙肝和重症乙肝患者血清代谢组差异。结果:与慢性乙肝患者血清代谢组比较,重肝患者血清中蛋氨酸、乙酰乙酸盐、三甲胺、葡萄糖和乳酸升高,而胆碱和脂的含量降低。结论:慢性乙肝和重症乙肝患者血清代谢组具有明显差异,基于核磁共振氢谱和主成分分析的代谢组学方法可以为重症乙肝的诊断提供代谢依据。
[关键词] 重症乙肝;代谢;核磁共振氢谱;主成分分析
[中图分类号] R373.2[文献标识码]A [文章编号]1673-7210(2010)06(c)-027-03
Metabonomic studies of serum obtained from chronic HBV and severe hepatitis B patients
YANG Yongxia1, CHEN Ali2, LIANG Minfeng3, QIU Cuihuan1, ZHANG Lei1
(1.Department of Physics and Electronic Teaching, Basic School of Guangdong Pharmaceutical University, Guangzhou 510006, China; 2.Central Lab of Zhongshan College of Guangdong Pharmaceutical University, Zhongshan 528458, China; 3.Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China)
[Abstract] Objective: To compare the metabolites of serum from chronic and severe HBV patients by using nuclear magnetic resonance 1H (1H-NMR) spectroscopy. Methods: 1H-NMR spectra were measured for serum samples from 20 chronic HBV patients and 11 severe HBV patients, and the spectra data were analyzed by principal component analysis (PCA) to reveal the metabonomic changes. Results: Obvious metabonomic differences existed between chronic HBV patients and severe HBV patients which were highlighted by the increased levels in methionine, acetoacetate, TMA, glucose and lactate together with the decreased levels of choline and lipids in severe HBV patients. Conclusion: The metabonomes of serum from severe HBV patients differ markedly from those of the chronic HBV patients, which indicates that serum 1H-NMR sepctroscopy in conjunction with PCA offers an efficient tool for understanding the biochemistry of severe hepatitis B.
[Key words] Severe hepatitis B; Metabonomics; Nuclear magnetic resonance; Principal component analysis
乙型肝炎病毒(hepatitis B virus,HBV)感染在全球广泛分布,慢性乙肝是导致肝硬化、肝功能衰竭和原发性肝癌的主要因素 ......
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