细胞外信号调节激酶蛋白在肺癌中的表达及意义(1)
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[摘要] 目的：检测细胞外信号调节激酶(ERK)在人肺癌和肺良性病变组织中的表达，探讨ERK蛋白与肺癌的组织类型、分化程度和淋巴结转移的关系。方法：选取77例肺癌标本和20例肺良性病变的组织，通过常规石蜡包埋行免疫组织化学染色，检测ERK2蛋白表达。结果：肺癌组和对照组ERK2蛋白表达的阳性率分别为51.9%和20.0%，两组间具有显著性差异(P<0.05)。ERK2蛋白表达在肺癌不同病理类型即鳞癌和腺癌中无显著性差异(P>0.05)。在高分化、中分化和低分化肺癌之间比较，ERK2表达阳性率有显著性差异(P<0.05)。ERK2在有无淋巴结转移中有显著性差异(P<0.05)。结论：肺癌组织中存在ERK2蛋白的高表达；ERK2蛋白的高表达与肺癌组织类型无关，而与分化程度和淋巴结转移有密切关系。因此，ERK2的表达与肺癌发生及发展的生物学行为和预后可能有关。
[关键词] 细胞外信号调节激酶-2；肺癌；免疫组织化学
[中图分类号] R734.2 [文献标识码]A [文章编号]1673-7210(2010)10(b)-015-03
Expression of ERK2 in lung cancer tissue and their biological significances
HE Xinrong, LIU Xin, HUANG Yifan, WEN Bin*
(Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China)
[Abstract] Objective: To examine the expression of extracellular signal-regulated protein kinase 2 (ERK2) in lung cancer tissue and biological significances. Methods: Immunohistochemistry was used to detect ERK2 expression in 77 human lung cancer tissues and 20 benign pulmonary disease tissues. Results: ERK2 immunoreactivity increased significantly from 20.0% in benign pulmonary lesion tissues to 51.9% in lung cancer tissues (P<0.05). In addition, the expression level of ERK2 protein increased significantly with the difference of the differentiation cancer (P<0.05). The ERK2 immunoreactivity increased significantly with lymph node metastasis of lung cancer (P<0.05). The over expression rates of ERK2 in adenocarcinoma and squamous cell carcinoma were not significantly different (P>0.05). Conclusion: An increased ERK2 expression is seen in lung carcinogenesis and metastasis. ERK2 may be associated with the carcinogenesis, biological behaviors and prognosis of lung cancer.
[Key words] Extracellular signal-regulated protein kinase 2; Lung cancer; Immunohistochemistry
细胞外信号调节激酶(extracellular signal-regulated kinase，ERK)是丝裂原活化蛋白激酶(mitogen actived protein kinase，MAPK)家族的一个亚族，其被多种生长因子和细胞因子激活后，参与细胞增殖、分化、转化、凋亡等。ERK是一种丝氨酸/苏氨酸蛋白激酶，它包括ERK1～7及最近发现的异构形式ERK50[1]。其中ERK1(P44MAPK)和ERK2(P42MAPK)是两个高度同源的亚型，均是MAPK家族中第一个被克隆的成员，其同源性接近85%，对其他ERK则了解较少。近年来研究表明，ERK1/2过度活化与肿瘤的发生有关[2] ......
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