脑梗死患者血清NO及tNOS\iNOS的测定(1)
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[摘要] 目的：通过观测正常人及脑梗死患者血清一氧化氮(NO)和一氧化氮合成酶(NOS)水平，探讨NO、NOS与脑梗死的关系。方法：用硝酸还原酶法测定血清NO。用NOS催化L-Arg和分子氧反应生成NO，NO与亲核性物质生成有色化合物后，再采用比色的方法测定血清NOS。测定42例脑梗死患者及18例健康人血清NO、tNOS和iNOS含量，分析上述指标的变化情况。结果：与健康对照组比较，脑梗死组NO含量明显降低(P<0.05)；tNOS及iNOS含量无显著性差异(P>0.05)。结论：血清NO、tNOS和iNOS参与了脑梗死的病理生理过程，其测定有助于对脑梗死患者的诊断及康复治疗。
[关键词] 脑梗死；一氧化氮(NO)；一氧化氮合成酶(NOS)
[中图分类号] R743[文献标识码]A [文章编号]1673-7210(2010)11(c)-009-02
Determination of serum nitric oxide (NO) and nitric oxide synthesis (tNOS, iNOS) in the patients with cerebral infarction
WU Xiaohua, YAN Jinhui, YANG Xiaohui
(Changzhou Medical College, Hebei Province, Changzhou 061001, China)
[Abstract] Objective: By observing serum nitric oxide (NO) and nitric oxide synthesis (NOS) levels of normal subjects and patients with cerebral infarction, to explore the relationship between NO, NOS and cerebral infarction. Methods: The levels of serum NO were determined by nitrate reductase. And with NOS catalytic L-Arg and molecular oxygen response to generate NO, NO and pro-nuclear substances to conduct a colored compound, then with colorimetric method determination of serum NOS. In 42 patients with cerebral infarction and 18 healthy volunteers, serum NO, tNOS and iNOS contents were determined and analysis of changes in these indicators. Results: Compared with the healthy control group, the serum NO decreased significantly in infarction group (P<0.05); tNOS and iNOS was no significant difference (P>0.05). Conclusion: Serum NO, tNOS and iNOS have involved in the pathophysiology of cerebral infarction, the determination could help the diagnosis of cerebral infarction and rehabilitation.
[Key words] Cerebral infarction; Nitric oxide (NO); Nitric oxide synthesis (NOS)
脑梗死发病与多种因素有关，由于缺血缺氧，脑组织产生一系列病理改变，引发“瀑布式”自由基连锁反应，产生大量自由基，造成广泛且严重的生物膜脂质过氧化，使膜通透性增加，结构遭到破坏，导致神经细胞、胶质细胞和血管内皮细胞损伤。目前认为自由基是参与脑缺血性损害发生的重要因素之一[1]。血管内皮功能障碍是多种心脑血管疾病的共同病理机制，其突出表现为内皮依赖性血管舒张功能障碍，主要由NO减少及氧自由基增加所致[2]。本文中笔者检测急性脑梗死患者血浆一氧化氮(NO)及一氧化氮合成酶(NOS)含量的变化，进一步了解NO及其合成酶在脑梗死急性期的变化规律，探讨其对脑梗死发病过程的病理生理意义，有助于对脑梗死患者的诊断及康复治疗。
1 资料与方法
1.1 一般资料 ......
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