HPLC法测定银杏叶软胶囊中总黄酮的含量(1)
 |
| 第1页 |
参见附件(1899KB，3页)。
[摘要] 目的：采用RP-HPLC法测定保健食品银杏叶软胶囊中总黄酮的含量。方法：采用Agilent C18(4.6 mm×250 mm，5 μm)色谱柱，流动相为甲醇-水(用磷酸调pH 为2.5 )(50∶50)，流速为1.0 ml/min，检测波长为360 nm，柱温为30℃。结果：槲皮素、山奈素、异鼠李素的线性范围分别为0.050～0.400、0.005～0.040、0.005～0.040 μg；3批样品中总黄酮含量分别为6.466、6.455、6.838 mg/粒。结论：本法简便、准确、分离效果好，适合于银杏叶软胶囊总黄酮的含量测定。
[关键词] 银杏叶软胶囊；总黄酮；槲皮素；山奈素；异鼠李素；含量测定
[中图分类号] R927.2 [文献标识码] A [文章编号]1673-7210(2010)11(c)-029-03
Determination of Total Flavonoid in Yinxingye Soft Capsules by HPLC
YE Xiubo1, CEN Guoying2, LI Ruiming2
(1.Traditional Chinese Medicine Hospital of Haizhu District, Guangzhou 510220, China; 2.Department of Pharmacy, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China)
[Abstract] Objective: To establish an RP-HPLC method for quantitative determination of Total Flavonoid in Yinxingye Soft Capsules. Methods: The analytical column was Agilent C18 (4.6 mm× 250 mm, 5 μm) column. The mobile phase was composed of methanol and water (with phosphoric acid pH=2) at a flow rate of 1.0 ml/min. The wavelength of UV detector was 360 nm and column temperature was 30℃. Results: The linear ranges of Quercetin, Kaempferol and Isorhamnetin were 0.050-0.400, 0.005-0.040, 0.005-0.040 μg, respectively. The contents of Total Flavonoid in three batches of samples were 1 Capsule 6.466, 6.455, 6.838 mg. Conclusion: This method is sensitive, repeatable and suitable to determine the content of Total Flavonoid in Yinxingye Soft Capsules.
[Key words] Yinxingye Soft Capsules; Total Flavonoid; Quercetin; Kaempferol; Isorhamnetin; Determination
银杏叶软胶囊是由银杏提取物与适量的基质制成的软胶囊，银杏提取物中含有黄酮类化合物和银杏总内酯两类生物活性物。其中，总黄酮类包括槲皮素、山奈素、异鼠李素等，共同作用于心脑和外周末梢血管系统，促进血液循环和改善末梢血液的运行，从而预防血管系统循环障碍所引起的各种症状。另外，黄酮类化合物有清除游离氧的抗氧化等药理作用[1]。本文用HPLC法测定总黄酮的含量，为建立银杏叶软胶囊质量控制方法提供依据。
1 仪器与试药
1.1 仪器
LC-2010高效液相色谱仪(日本岛津公司)；KQ-300超声清洗仪(昆山市超声仪器有限公司)；色谱柱Agilent C18(4.6 mm×250 mm，5 μm)。
1.2 试药
槲皮素对照品(供含量测定用，批号：10081-200406，中国药品生物制品检验所)；山奈素对照品(供含量测定用，批号：110861-200303，中国药品生物制品检验所)；异鼠李素对照品(供含量测定用，批号：110860-200205，中国药品生物制品检验所)；甲醇为色谱纯，水为重蒸馏水，其他试剂均为分析纯，银杏叶软胶囊(批号：080510、080920、091026，广州美丽康有限公司)。
2 方法与结果
2.1 色谱条件
采用Agilent C18(4.6 mm×250 mm，5 μm)色谱柱，流动相为甲醇-水(50∶50)(用磷酸调pH为2.5)，流速为1.0 ml/min，检测波长为360 nm[2] ......
您现在查看是摘要介绍页，详见PDF附件(1899KB，3页)。