两种不同的上游法处理精液对常规体外受精结果的影响(1)
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[摘要] 目的：探讨使用两种不同的上游法分离的精子对常规体外受精(in-vitro fertilization，IVF)结果的影响。方法：本研究共287个周期，将患者夫妇，女方卵子分为约等的两份，男方精液分为等量的两份，并分别使用两种上游法优选精子，并将分离的精子与相应卵子行常规体外受精操作。观察两组受精卵的受精情况，并将受精卵的受精率、卵裂率、优质胚胎率作分析比较。结果：先离心组完全受精失败14个周期，受精率为72.4%，卵裂率为91.2%，优质胚胎率为52.5%；先上游组完全受精失败12个周期，受精率为75.1%，卵裂率为93.2%，优质胚胎率为53.9%，各对照指标未发现差异有统计学意义(P>0.05)。结论：此两种上游方法对于受精结果的影响虽无统计学意义，但结合相关文献报道，先上游法应优于先离心法。
[关键词] 常规体外受精；上游法；受精失败
[中图分类号] R715.2[文献标识码] B [文章编号] 1673-7210(2010)11(c)-046-02
The results of fertilization with two different sperm swimming-up methods
ZHANG Hong1, MA Bo2
(1.Department of Postgraduate, Guangxi Medical University, Nanning 530021, China; 2.Reproductive Center of Police Hospital, Shenzhen 518029, China)
[Abstract] Objective: To compare the results of in-vitro fertilization with two different swim-up methods to separate sperm and co-culture with oocytes. Methods: Retrospective analysis 287 cycles receiving IVF treatment in our center from February 2009 to September 2009, sperm collected from patients were divided into two parts, one was separated with swim-up first method, another was separated with centrifuge first method. Then, co-culturing the treated sperm with the matched oocytes. The differences of fertilization results between the two groups were compared. Results: In the centrifuge first group, 14 cycles were fertilization failure, fertilization rate was 72.4%, the cleavage rate was 91.2%, high quality embryo rate was 52.5%. In the swim-up first group, 12 cycles were fertilization failure, fertilization rate was 75.1%, the cleavage rate was 93.2%, high quality embryo rate was 53.9%. All the results in swim-up first group were better than the ones in the centrifuge first group, however, there was no statistical difference between the two groups (P>0.05). Conclusion: the sperm, which had been separated by the two different swim-up methods, did not effect the in-vitro fertilization results significantly.
[Key words] In-vitro fertilization; Swim-up; Fertilization failure
上游法是较早用于分离精子的技术之一，由于其操作简便也是现在多数生殖实验室使用的精子优选方法[1] ......
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