心肌肥厚大鼠外周血CGRP\NPY和IL-6的表达(1)
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[摘要] 目的：探讨神经肽-Y(NPY)、降钙素基因相关肽(CGRP)、白介素-6(IL-6)在心肌肥厚大鼠外周血的表达变化及意义。方法：将Wistar大鼠20只随机分成两组，心肌肥厚组(OG组)和假手术组(CG组)，每组10只。参照Anderson方法制作压力负荷性心肌肥厚大鼠模型，采用放射性免疫法检测大鼠外周血NPY、CGRP、IL-6的浓度。结果：与对照组比较，心肌肥厚组NPY、IL-6的水平升高(t=4.52，P<0.05；t=4.73，P<0.05)，与心重指数呈正相关(r=0.64，P<0.05；r=0.60，P<0.05)； CGRP水平降低(t=2.99，P<0.05)，与心重指数呈负相关(r=-0.65，P<0.05)。结论：NPY、CGRP和IL-6等因子参与心肌肥厚的发生与发展。
[关键词] 心肌肥厚；降钙素基因；相关肽神经肽-Y；白介素-6
[中图分类号] R542.2 [文献标识码]A [文章编号]1673-7210(2011)04(a)-028-03
NPY, CGRP and IL-6 changes in peripheral blood of heart hypertrophy rat
SHI Jun1, WANG Jing2*, WANG Xing1
1.Inner Mongolia Medical College, Inner Mongolia, Huhhot 010059, China; 2.Department of Orthopaedics, the Affiliated Hospital of Inner Mongolia Medical College, Inner Mongolia, Huhhot 010059, China
[Abstract] Objective: To study the expression of neuropeptide-Y (NPY), calcitonin gene-related peptide (CGRP) and Interleukin-6 (IL-6) in peripheral blood and in the hypertrophic myocardium of rat. Methods: 20 Wistar rats were divided into 2 groups and one group contained 10 rats. The first group was myocardial hypertrophic group(OG group), the second was control group (CG group). The partial abdominal aorta ligature was used to induce hypertrophy in rats. NPY, CGRP and IL-6 were determined with radio immunological assay. Results: Compared with the CG group, in the OG group, myocardial hypertrophy NPY, IL-6 levels increased (t=4.52, P<0.05; t=4.73, P<0.05), and heart weight index was positively correlated (r=0.64, P<0.05); CGRP levels decreased (t=2.99, P<0.05), and heart weight index was negatively correlated (r=-0.65, P<0.05). Conclusion: The level of NPY, CGRP and IL-6 are related with the development of left ventricular hypertrophy.
[Key words] Hypertrophy; Calcitonin gene-related peptide; Neuropeptide Y; Interleukin-6
心肌肥厚是多种心血管疾病的共同病理过程，其发生的结构基础主要是心肌细胞增大和纤维化。实验表明[1]，压力负荷是导致心肌肥厚的主要因素，但是其具体机制没有完全明了。降钙素基因相关肽(CGRP)、神经肽-Y(NPY)是由心血管系统肽能神经纤维分泌的神经递质，过去对这些递质在心血管功能的调节作用研究较多，而对其在心肌肥大形成过程中的作用及可能机制并不清楚。白介素-6(IL-6)是由多种细胞分泌的细胞因子，是免疫以及炎症反应的重要调节因子，近年来研究发现它与心血管疾病关系密切，但是关于IL-6在心肌肥厚过程中变化的报道较少，而且神经递质CGRP、NPY与细胞因子IL-6在心肌肥厚的发生、发展中有无交互作用尚未阐明。本实验采用放射免疫方法 ......
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