咪达唑仑与丙泊酚对小鼠学习记忆能力的影响(1)
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[摘要] 目的：研究咪达唑仑和丙泊酚对小鼠学习记忆能力的影响。方法：将88只小鼠随机分为8组，每组各11只，分别为：咪达唑仑(腹腔注射15、30、60 mg/kg)组,丙泊酚(腹腔注射60、120、240 mg/kg)组，生理盐水(腹腔注射10 ml/kg)组，和一组不作任何处理的空白对照组。然后用自建的水迷宫测试其对小鼠学习记忆能力的影响。结果：小鼠第一次注射,恢复正常后马上进行水迷宫实验，与注射前相比，咪达唑仑三组潜伏期均延长(其中15 mg/kg组t=2.693，P=0.023；30 mg/kg组t=4.633，P=0.001；60 mg/kg组t=2.550，P=0.029)，其他各组潜伏期无显著性差异(P>0.05)。继续注射两天后停药24 h，再进行一次水迷宫实验，与第一次注射前相比，丙泊酚120 mg/kg和240 mg/kg组潜伏期缩短(分别为t=2.447，P=0.034；t=4.619，P=0.001)，其他各组潜伏期无明显差异(P>0.05)。结论：咪达唑仑对小鼠的学习记忆能力影响具有瞬时性，不具长期性；丙泊酚对小鼠学习记忆能力的影响不具瞬时性，影响时间较长；并且未发现两药的影响程度与剂量具有明显梯度关系。
[关键词] 咪达唑仑；丙泊酚；自建水迷宫；学习记忆能力
[中图分类号] R965[文献标识码] A [文章编号]1673-7210(2011)04(a)-062-03
Effect of Midazolam and Propofol on learning memory of mice
CHEN Yanfang, WU Weipeng, WANG Yuping, CHEN Jinwen
Fujian Medical University，Fuzhou 350108，China
[Abstract] Objective: To observe the effect of Midazolam and Propofol on learning memory of mice. Methods: 88 mice were divided into 8 groups, each group had 11 mice: Midazolam group(15,30,60 mg/kg, intraperitoneal injection), Propofol group(60,120,240 mg/kg, intraperitoneal injection),normal saline group(10 ml/kg, intraperitoneal injection), and blank control group. Then using self-built water maze to test the effect of medicine on learning memory of mice. Results: The first time after medication, as soon as they backed to normal, tested them and found out the latency of all the Midazolam groups was prolonged(15 mg/kg: t=2.693，P=0.023；30 mg/kg: t=4.633，P=0.001；60 mg/kg: t=2.550，P=0.029).There was no difference among other groups(P>0.05).Keeping on injecting two days,24 hours later, test them again and find out that the latency of Propofol groups(120,240 mg/kg)was reduced(120 mg/kg: t=2.447,P=0.034;240 mg/kg: t=4.619,P=0.001).There was no difference among other groups(P>0.05). Conclusion: The effect of Midazolam is instantaneous other than persistent. While the effect of Propofol is opposite, and there is no clear relationship between the degree of influence and dose.
[Key words] Midazolam; Propofol; Self-built water maze; Learning memory
全身麻醉药除了具有镇静、催眠的作用外，还具有遗忘作用，这是导致术后认知功能障碍的重要原因之一，咪达唑仑和丙泊酚是目前临床最常用的全身麻醉药物，通过控制其他外部因素(手术、激光等)的影响，进行动物实验深入研究它们对学习记忆能力的影响，为临床合理应用全麻药物提供指导，从而减少术后认知功能障碍的发生。
1 材料与方法 ......
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