山核桃树叶中球松素查耳酮的含量测定(1)
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[作者简介] 马哲龙(1986-)，男，硕士研究生；研究方向：中药化学。
[通讯作者] 丁志山(1967-)，男，教授，博士；研究方向：中药化学。
[摘要] 目的：建立用高效液相色谱法(HPLC)法测定山核桃树叶中球松素查耳酮(DHMC)含量的方法。方法：采用Kromasil C18(250 mm×4.6 mm，5 μm)色谱柱，以甲醇-水(65∶35，V/V)为流动相，柱温为30℃，流速为1.0 ml/min，检测波长为342 nm，对DHMC的含量进行测定。结果：DHMC进样量在0.02～0.10 μg范围内线性关系良好(r=0.999 6，n=6)，精密度RSD为1.1%(n=6)，重复性试验RSD为0.9%(n=6)，加样回收率为101.6%(RSD=1.8%，n=6)，经测定山核桃叶干品中DHMC的含量较高，达0.79%。结论：所建立的HPLC法专属性强，精密度好，结果准确。
[关键词] 山核桃叶；球松素查儿酮；高效液相色谱法；含量测定
[中图分类号] R482.2 [文献标识码] A[文章编号]1673-7210(2011)08(a)-069-02
Determination on the content of DHMC from the leaf of Carya cathayensis Sarg.
MA Zhelong1, JIANG Fusheng2, YU Tingting2, YU Yan3, CHEN Jianzhen1, DING Zhishan2
1.College of Pharmaceutical, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China; 2.College of Life and Science, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China; 3.College of the First Clinical Medicine, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
[Abstract] Objective: To establish the HPLC method for the determination on the content of DHMC in the Carya cathayensis Sarg.. Methods: The Kromasil C18 (250 mm×4.6 mm,5 μm) column was used for the determination on the content of DHMC with mobile phase consisted of methanol and water (65:35, v/v). Column temperature was at 30℃, flow velocity was 1.0 ml/min, the detective wavelength was at 342 nm. Results: The standard curves of DHMC was linear in the concentration range of 0.02-0.10 μg (r=0.999 6, n=6). The precision RSD was 1.1%(n=6), the repeatability RSD was 0.9% (n=6). The average recovery was 101.6% (RSD=1.8%, n=6), the content of DHMC in Carya cathayensis Sarg. was 0.79%. Conclusion: This method is simple and accurate with good reproducibility.
[Key words] The leaf of Carya cathayensis Sarg.; DHMC; High Performance Liquid Chromatography; Content determination
山核桃(Carya cathayensis Sarg.)为胡桃科山核桃属植物，主要分布于浙、皖两省交界的天目山区周围，尤以临安、昌化为佳，具有明显的地域分布特性[1]。山核桃具有广泛的药理作用，山核桃树皮具有清热解毒、抗菌消炎、镇痛、抗氧化、抗肿瘤的作用，山核桃外果皮具有抗炎镇痛、抑菌消炎的作用，山核桃叶片提取物有很好的抑菌作用，对金黄色葡萄球菌、大肠杆菌、酵母菌、黄曲霉菌、青霉菌和黑曲霉菌均有效[2-7] ......
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