6,8-二-三氟甲基-7-乙酰氧基白杨素抑制人肝癌裸鼠移植瘤生长(1)
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[摘要] 目的：研究6,8-二-三氟甲基-7-乙酰氧基白杨素(dFMAChR)对人肝癌裸鼠移植瘤生长的抑制作用。方法：建立人肝癌HepG2细胞裸鼠移植瘤模型，随机分为6组，生理盐水(0.1 ml/10 g)组、氟尿嘧啶(5-Fu，20 mg/kg)组、白杨素(100 mg/kg)组、低剂量dFMAChR(20 mg/kg)组、中剂量dFMAChR(40 mg/kg)组和高剂量dFMAChR(80 mg/kg)组。测定移植瘤大小和重量；HE染色，光镜下观察移植瘤组织形态学特征；TUNEL法检测移植瘤细胞凋亡作用。结果：20、40、80 mg/kg dFMAChR 诱导的移植瘤的瘤重抑制率分别为40.17%、47.41%和66.81%。HE染色与TUNEL法检测结果表明，dFMAChR能明显诱导移植瘤细胞凋亡。结论：dFMAChR具有抗人肝癌治疗作用。
[关键词] 肝癌；白杨素；6，8-二-三氟甲基-7-乙酰氧基白杨素；移植瘤
[中图分类号] R735.7 [文献标识码] A [文章编号] 1673-7210(2011)11(b)-005-03
Inhibition of growth of human liver carcinoma xenograft tumor by 6, 8-ditrifluoromethy-7-acetoychrysin in nude mice
TAN Xiangwen1, XIA Hong2, XU Jinhua3, CAO Jianguo4*
1.Department of Laboratory Animal Science, University of South China, Hengyang 421001, China; 2.Institute of Cancer Research, University of South China, Hengyang 421001, China; 3.Laboratory Center of Biochemistry and Molecular Biology, University of South China, Hengyang 421001, China; 4.College of Medicine, Hu'nan Normal University, Changsha 410006, China
[Abstract] Objective: To investigate the inhibitory effects of 6, 8-ditrifluoromethy-7-acetoychrysin (dFMAChR) on the growth of human liver carcinoma xenograft tumor in nude mice. Methods: The nude mice model was established by inoculation of human liver carcinoma HepG2 cells, and randomly divided into six groups, NS group (0.1 ml/10 g), 5-FU (20 mg/kg) group, ChR (100 mg/kg) group, low dose dFMAChR (20 mg/kg) group, media dose dFMAChR (40 mg/kg) group and high dose dFMAChR (80 mg/kg) group. The body weight and tumor volume and weight were investigated. The histomorphology change of the xenografts were observed by HE staining. TUNEL staining was utilized to observe the apoptosis of human liver cancer xenograft cells in nude mice. Results: The inhibition rate of tumor weight in mice treated with dFMAChR at a dose of 20, 40, 80 mg/kg was 40 ......
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