NKX2.5 基因修饰骨髓间充质干细胞对心肌梗死大鼠心功能的影响(1)
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[摘要] 目的：以NKX2.5融合绿色荧光蛋白基因(NKX2.5-pEGFP)质粒转染大鼠骨髓间充质干细胞(MSCs)，将基因修饰后MSCs移植入大鼠心肌梗死模型中，评价其对心功能的影响。方法：体外分离培养大鼠MSCs，构建NKX2.5-pEGFP基因质粒，以lipo2000将质粒转染入MSCs。将基因修饰后MSCs植入心肌梗死后10 d的大鼠心肌梗死区(MSCs组，n=20)，同时将注射等体积无血清改良依格尔(DMEM)培养液于心肌梗死区的大鼠作为DMEM组(n=20)。移植注射后4周，通过检测左心室射血分数(LEVF)、左室缩短分数(FS)等研究其对心功能的影响。结果：移植4周后，MSCs组大鼠LEVF[(79±4)%]较DMEM组[(47±3)%]显著提高(P<0.01), MSCs组大鼠FS[(48±9)%]较DMEM组[(25±6)%]显著提高(P<0.01)。结论：移植NKX2.5基因修饰后的MSCs能够改善心肌梗死大鼠心功能。
[关键词] NKX2.5基因；骨髓间充质干细胞；心肌梗死；心功能
[中图分类号] R541 [文献标识码] A [文章编号] 1673-7210(2011)11(b)-018-03
Heart function effect of mesenchymal stem cells modified by NKX2.5 in infracted myocardium in rats
ZHAO Donghai1, ZHANG Lei2, LI Dapeng3
1.Department of Pathology, Jilin Medicine and Pharmacy College, Jilin Province, Jilin 132013, China; 2.Department of Biochemistry and Inspection, Jilin Medicine and Pharmacy College, Jilin Province, Jilin 132013, China; 3.Department of Respiratory Medicine, the Affiliated Hospital of Jilin Medcine and Pharmacy College, Jilin Province, Jilin 132013, China
[Abstract] Objective: To transfect NKX2.5-pEGFP plasmid into mesenchymal stem cells (MSCs) and transplant the gene modified MSCs in rat myocardial infarction model to estimate the effect on heart function. Methods: MSCs were isolated culture in vitro. NKX2.5-pEGFP plasmid was constructed and transfected into MSCs. The gene modified MSCs was transplanted in the area of rat myocardial infarction model after 10 days (MSCs group, n=20). The equal volume of none blood serum DMEM was injected in myocardial infarction model as DMEM group (n=20). The effect on heart function was estimated by detecting left ventricular ejection fraction (LVEF) and fractional shortening (FS) after injecting 4 weeks. Results: 4 weeks after transplantation, the LEVF value of MSCs group [(79±4)%] increased significantly compared to that of DMEM group [(47±3)%] (P<0.01) and the FS value of MSCs group [(48±9)%] increased significantly compared to that of DMEM group [(25±6)%] (P<0 ......
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