尿培养病原菌分布及耐药性分析(1)
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[摘要] 目的：分析泌尿系统感染尿标本培养的病原菌分布及耐药性。方法：对我院2007年1月~2010年12月泌尿系感染患者的中段尿进行分离培养，病原菌的鉴定及药敏试验采用珠海迪尔公司的黑马全自动分析仪进行操作，取纯菌落稀释后加入试验板中培养18~24 h后由全自动分析仪鉴定及报告药敏结果。结果：4年共分离菌株1 161例，其中，大肠埃希菌占31.2%，真菌占14.2%，肠球菌属占10.4%，肺炎克雷伯菌占9.4%，金黄色葡萄球菌占7.8%，绿脓假单胞菌占5.9%，其他菌属占21.2%。菌株耐药率呈现较高的状况。结论：泌尿系感染病原菌种类较多，耐药率较高，应引起临床高度重视。
[关键词] 泌尿系统感染；耐药性；耐药率；病原菌
[中图分类号] R446.12 [文献标识码] B [文章编号] 1673-7210(2011)11(c)-092-03
Analysis on pathogenic bacteria distribution in urine culture and drug resistance
ZHANG Hao, LI Rui
The Fifth People's Hospital of Shenyang City, Liaoning Province, Shenyang 110023, China
[Abstract] Objective: To analyze pathogenic bacteria distribution and drug resistance with urine samples from urinary tract infection. Methods: Urine of patients with urinary tract infection in our hospital were cultured from January 2007 to December 2010, both pathogenic bacteria testing and durg susceptibility testing were tested by using Heima Automatic analyzer of Zhuhai dill company, and then pure colonies were diluted, and they were put onto the test plate for 18 to 24 hours, finally the results of species and reported of drug susceptibility were identified by the automatic analyzer. Results: A total of 1 161 strands pathogens were isolated in 4 years, Escherichia coli accorded for 31.2%, fungus accorded for 14.2%, enterococcus accorded for 10.4%, Klebsiella pneumonia accorded for 9.4%, staphylococcus aureus accorded for 7.8%, pseudomonas aeruginosa accorded for 5.9%, the other legionella accorded for 21.2%. The drug resistance of resistant strains showed higher rates. Condusion: Urinary tract infection has more pathogen species and the higher drug resistance rate, which can lead to clinical high attention.
[Key words] Urinary tract infection; Drug resistance; Drug resistance rate; Pathogenic bacteria
泌尿系统感染是临床常见的感染性疾病，同时也是院内感染的重要疾病之一，由于临床医生对广谱抗生素的大量应用，使得细菌的耐药性日益增高，抗生素的选择越来越困难。现对我院尿培养阳性标本的菌株及其耐药性进行分析，以期为临床用药提供帮助。
1 材料与方法
1.1 材料
1 161例菌株来自于我院2007年1月~2010年12月泌尿系感染患者尿培养的阳性标本，嘱其在应用抗生素之前，清洗外阴，以无菌手法留取中段尿。
1.2 试验方法
标本的接种与分离依照《全国检验操作规程第三版》[1]进行操作 ......
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