肝细胞癌中HBx蛋白与生存素蛋白表达的关系及其意义(1)
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[摘要] 目的：探讨乙型肝炎病毒X蛋白(HBx)与生存素在肝细胞癌(HCC)表达发生中的作用。方法：采用免疫组织化学测定58例患者的HCC组织中HBx蛋白和生存素蛋白表达，用含有辣根过氧化物酶底物的试剂合(DAB)显色，已知的肝癌组织HBx及生存素阳性切片为阳性对照，磷酸盐缓冲液(PBS)作为空白对照。结果：HCC组织HBx和生存素的表达率分别为46.6%(27/58)和69.0%(40/58)，HBx蛋白阳性的HCC组织中生存素蛋白阳性表的阳性率为70.4%(19/27)，HBx蛋白的阴性的HCC组织中生存素蛋白阳性表的阳性率为27.5%(11/40)，差异有统计学意义(P<0.05)；HBx蛋白与生存素蛋白表达与HCC分化程度、转移及甲胎蛋白(AFP)相关。结论：在HCC的发生过程中HBx蛋白可上调生存素蛋白的表达，促进肝细胞癌的发生发展。
[关键词] 肝细胞癌；乙型肝炎病毒X蛋白；肝炎病毒；生存素；凋亡
[中图分类号] R735.7 [文献标识码] A [文章编号] 1673-7210(2011)12(b)-022-03
The significance and relationship between HBx protein and survivin portein expression in hepatic cellular cancer
CHEN Jiang, CUI Bin, JIANG Hanguo
Department of Oncology, the people's Hospital of Lianjiang City, Guangdong Province, Lianjiang 524400, China
[Abstract] Objectve: To investigate the effect of HBx protein and survivin protein in the expression of hepatic cellular cancer. Methods: The expression of HBx and survivin protein in hepatic cellular cancer tissues of 58 patients were detected by immunohistochemistry method, the protein was colorated by DAB, the positive pathological section of HBx and survivin in hepatic cellular cancer was taken as positive control, while PBS was taken as blank control. Results: The positive rates of HBx and survivin were 46.6% (27/58) and 69.0% (40/58) respectively. The positive rate of survivin in HBx-positive group was 70.4% (19/27) and 27.5% (11/40) in HBx-negative group, the differences were significant (P<0.05). The expression of survivin protein and HBx protein was significantly related with differentiation of hepatic cellular cancer, metastasis and serum alpha-fetoprotein (AFP) level. Conclusion: HBx protein can promote the expression of survivin protein and the development of hepatic cellular cancer in the carcinogenesis of hepatic cellular cancer.
[Key words] Hepatic cellular cancer; HBx; Hepatitis virus; Survivin portein; Apoptosis
乙型肝炎病毒感染影响到全球近4亿人口，是肝细胞癌(hepatic cellular cancer，HCC)最重要的独立危险因素。乙型肝炎病毒是3.4 kb的DNA病毒，含有4个部分双链的开放读码框，编码C、S和X蛋白及病毒DNA聚合酶 ......
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