球形红细菌生物转化槲寄生中总三萜类化合物的测定(1)
 |
| 第1页 |
参见附件。
[摘要] 目的：建立槲寄生及球形红细菌转化槲寄生培养液中总三萜类化合物的含量测定方法，并对各个样品的含量进行比较研究。方法：采用紫外分光光度法，以齐墩果酸为对照品，5%的香草醛-冰醋酸溶液和高氯酸为显色系统，535 nm波长处测定总三萜类化合物的含量。结果：齐墩果酸在2～12 μg/ml的范围内线性关系良好，标准曲线回归方程为A=21.988C-0.072，r=0.989 5。75%的乙醇提取、半量常规培养基、游离球形红细菌培养，所得培养液(样品3)总三萜含量和槲寄生75%的乙醇提取液(样品1)相比增加141%。结论：经过球形红细菌转化可以增加槲寄生提取液中总三萜类化合物的含量。
[关键词] 槲寄生；光合细菌；球形红细菌；三萜类化合物；含量测定
[中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210(2011)12(b)-029-03
Determination of the total triterpenes in mistletoe transformed by rhodobacter sphaeroides
HOU Xiaofeng1, ZHENG Qinghong1, QI Xiaomei1, YANG Guan′e1*, ZHANG Zhaoming2
1.College of Pharmacy, Shanxi Medical University, Shanxi Province, Taiyuan 030001, China; 2.College of Life Science and Technology, Shanxi University, Shanxi Province, Taiyuan 030006, China
[Abstract] Objective: To establish methods for the determination of total triterpenes in mistletoe and its transformation products by rhodobacter sphaeroides, and to compare the contents of each sample. Methods: Oleanolic acid was taken as the standard, the content of total triterpenes was measured by spectrophotometry with 5% vanillin-glacial acetic acid solution and perchloric acid as color-developing agent and the detection wavelength was at 535 nm. Results: The linear correlation of oleanolic acid calibration curve was good in the range of 2-12 μg/ml, and the calibration curve regression equation was A=21.988C-0.072, r=0.989 5. Compared with 75% ethanol extract of mistletoe (sample 1), the content of total triterpenes in 75% ethanol mistletoe extract cultured with half amount of conventional medium and rhodobacter sphaeroides (sample 3) increased by 141%. Conclusion: The content of total triterpenes in mistletoe extract can be enhanced after bio-transformed by rhodobacter sphaeroides.
[Key words] Mistletoe; Photosynthetic bacteria; Rhodobacter sphaeroides; Triterpenes; Content determination
槲寄生为桑寄生科槲寄生属植物V. coloratum (Komar.)Nakai 的干燥带叶茎枝，具有祛风湿、补肝肾、强筋骨、安胎作用[1]。其中含有黄酮类、生物碱类、三萜类、多肽等化合物 ......
您现在查看是摘要介绍页，详见PDF附件(2484kb)。